TY - JOUR
T1 - Prednisolone sodium succinate down-regulates BSAP/Pax5 and causes a growth arrest in the Nalm6 pre-B cell line
AU - Rahman, Mizanur
AU - Hirabayashi, Yasuhiko
AU - Ishii, Tomonori
AU - Watanabe, Miki
AU - Maolin, Liu
AU - Sasaki, Takeshi
PY - 2001/3
Y1 - 2001/3
N2 - Glucocorticoids (GCs) induce growth arrest and apoptosis of B lineage cells, especially pre-B and immature B cells, but little is known about this mechanism. Since B cell-specific activator protein (BSAP) encoded by the Pax5 gene is an essential transcription factor for B-cell proliferation, we have investigated the effect of prednisolone sodium succinate (Pss) on BSAP expression in several human leukemic B lineage cell lines. Pss caused growth arrest of Nalm6 (pre-B) cells, but this is not the case for 207 (pro-B) and Raji (mature B) cells. In Nalm6 cells, the growth rate and the amount of BSAP bound to its recognition sequence decreased in proportion to the Pss dose. Pss also reduced the expression level of BSAP as well as of Pax5 mRNA. These data suggest that the suppression of Pax5/BSAP may be involved in the GCs-induced growth arrest of pre-B cells.
AB - Glucocorticoids (GCs) induce growth arrest and apoptosis of B lineage cells, especially pre-B and immature B cells, but little is known about this mechanism. Since B cell-specific activator protein (BSAP) encoded by the Pax5 gene is an essential transcription factor for B-cell proliferation, we have investigated the effect of prednisolone sodium succinate (Pss) on BSAP expression in several human leukemic B lineage cell lines. Pss caused growth arrest of Nalm6 (pre-B) cells, but this is not the case for 207 (pro-B) and Raji (mature B) cells. In Nalm6 cells, the growth rate and the amount of BSAP bound to its recognition sequence decreased in proportion to the Pss dose. Pss also reduced the expression level of BSAP as well as of Pax5 mRNA. These data suggest that the suppression of Pax5/BSAP may be involved in the GCs-induced growth arrest of pre-B cells.
KW - BSAP
KW - Glucocorticoids
KW - Growth arrest
KW - Pax5
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U2 - 10.1620/tjem.193.237
DO - 10.1620/tjem.193.237
M3 - Article
C2 - 11315771
AN - SCOPUS:0035003597
SN - 0040-8727
VL - 193
SP - 237
EP - 244
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
IS - 3
ER -