Poly-N-isopropylacrylamide (PNIPA) cryogels containing liposomes were prepared by polymerization under freezing conditions at sub-zero temperature. The cryogel had a highly porous structure with 20–30 μm pore size. The wall thickness of the cryogel containing liposomes was larger than that without liposomes. The structural change and aggregation of the liposomes in the cryogel were observed. The immobilization of the phospholipid and the dynamic property of the lipid membrane in the cryogel were confirmed. The cryogel containing liposomes showed chiral recognition ability toward L-Trp. The selective adsorption of L-Trp was achieved with the PNIPA cryogel containing liposome.