Preparation and in vivo evaluation of apatite/collagen packed composite by alternate immersion method and Newton press

Masayuki Taira, Takashi Nezu, Kaori Sasaki, Setsuo Saitoh, Tadayoshi Kagiya, Hidemitsu Harada, Yukyo Takada, Yoshima Araki

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8 Citations (Scopus)


Further development of bio-compatible, bio-absorbable, and osteo-conductive biomaterials is desired for bone grafts in dental and medical clinics. One candidate material might be a high-density apatite/collagen composite, which cures relatively large bone defects. To produce such a composite, we freeze-dried type I collagen solution, cross-linked the formed sponge by 2 wt % glutaraldehyde, immersed the insoluble sponge in CaCl2 and Na 2HPO4 solutions alternately five times, and compacted the sponge by Newton press at 5000 kgf. For comparison, cross-linked collagen without alternate immersion was also pressed. SEM/EPMA, XRD, and FTIR analyses clarified that alternate immersion successfully coated the collagen sponge with hydroxyapatite. Packed apatite/collagen composite and collagen disks 6 mm in diameter and 0.5 mm in height were implanted in the subperiostea of rabbit tibiae for 2, 4, 8, and 12 weeks to assess bio-compatibility, bio-absorbability, and osteo-conductivity. Histological observations showed that the packed apatite/collagen composite was biocompatible, osteo-conductive for up to 8 weeks, and largely bio-absorbed at 12 weeks, while the packed collagen sponge caused an undesirable foreign body reaction, which worsened with the implantation period. The overall findings suggest that this packed apatite/collagen composite might be used as a new bio-absorbable bone graft material.

Original languageEnglish
Pages (from-to)566-573
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume90 B
Issue number2
Publication statusPublished - 2009 Aug


  • Calcium phosphate(s)
  • Collagen
  • Composite/hard tissue
  • Dental/craniofacial material
  • Foreign body reactions (response)


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