Preparation of liposomes encapsulating β–carotene using supercritical carbon dioxide with ultrasonication

Yukako Tanaka, Chiho Uemori, Tatsuhiko Kon, Masaki Honda, Wahyudiono, Siti Machmudah, Hideki Kanda, Motonobu Goto

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23 Citations (Scopus)


β –Carotene-loaded liposomes were prepared utilizing ultrasonication and supercritical carbon dioxide (SC-CO2) as an alternative to organic solvents. Thermally Z–isomerized β–carotene samples containing 2.4, 37, or 87 % Z–isomers were fed with hydrogenated soy phosphatidylcholine. Pressure and temperature were changed in ranges 10–25 MPa and 50–70 °C, respectively, and isomerization ratio was changed. Transmission electron microscopy revealed ∼90–150 nm spherical liposomes. Ultraviolet–visible spectroscopy indicated that β–carotene aqueous nanosuspensions formed more effectively when Z–isomer-rich β–carotene was used as a starting material. Zeta potentials indicated that soy-phosphatidylcholine-based Z–isomer-containing β–carotene aqueous nanosuspensions showed good stability.

Original languageEnglish
Article number104848
JournalJournal of Supercritical Fluids
Publication statusPublished - 2020 Jul 1


  • Liposome
  • Supercritical carbon dioxide
  • Ultrasonication
  • Z–isomerization
  • β–Carotene


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