Abstract
Multilayer films comprising of glucose oxidase (GOx) and phenylboronic acid-modified poly(amidoamine) dendrimer (PBA-PAMAM) were prepared and their pH- and glucose-induced decomposition was studied. Multilayered GOx/PBA-PAMAM films were prepared through boronate ester linkages between PBA-PAMAM and carbohydrate chains of GOx. In contrast, preparation of multilayer film was unsuccessful when unmodified PAMAM was used in lieu of PBA-PAMAM, confirming an essential role of PBA residues in constructing multilayer films. The ten-layered (GOx/PBA-PAMAM)10 film was stable in aqueous solutions of pH 8.5, whereas the multilayer film decomposed in neutral and acidic media as a result of cleavage of the boronate ester bond. The pH threshold for the film decomposition was pH 7.0–8.0. In addition, the (GOx/PBA-PAMAM)10 film decomposed in glucose solutions owing to the oxidative scission of carbon–boron bond of PBA-PAMAM by enzymatically produced hydrogen peroxide. Thus, the potential use of the (GOx/PBA-PAMAM)10 film for the development of pH- and glucose-sensitive devices is suggested.
| Original language | English |
|---|---|
| Pages (from-to) | 2713-2718 |
| Number of pages | 6 |
| Journal | Colloid and Polymer Science |
| Volume | 293 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2015 Sept 13 |
Keywords
- Dendrimer
- Glucose oxidase
- Multilayer film
- Phenylboronic acid
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Polymers and Plastics
- Colloid and Surface Chemistry
- Materials Chemistry