Preparative, electroenzymatic reduction of NAD+ to NADH on a thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen-cation-exchange polymer and diaphorase

Yoshitomo Kashiwagi; Yoshinori Yanagisawa; Norihiko Shibayama; Kaname Nakahara; Futoshi Kurashima; Jun Ichi Anzai; Tetsuo Osa

doi:10.1246/cl.1996.1093

Preparative, electroenzymatic reduction of NAD⁺ to NADH on a thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen-cation-exchange polymer and diaphorase

Yoshitomo Kashiwagi, Yoshinori Yanagisawa, Norihiko Shibayama, Kaname Nakahara, Futoshi Kurashima, Jun Ichi Anzai, Tetsuo Osa

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen - cation-exchange polymer and diaphorase was prepared and applied to preparative electroenzymatic reduction of NAD⁺ in phosphate buffer at constant potential of - 0.80 V vs. SCE. The electrode was stable for the reaction and yielded NADH quantitatively in 97.8% current efficiency.

Original language	English
Pages (from-to)	1093-1094
Number of pages	2
Journal	Chemistry Letters
Issue number	12
DOIs	https://doi.org/10.1246/cl.1996.1093
Publication status	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.1996.1093

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Kashiwagi, Y., Yanagisawa, Y., Shibayama, N., Nakahara, K., Kurashima, F., Anzai, J. I., & Osa, T. (1996). Preparative, electroenzymatic reduction of NAD⁺ to NADH on a thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen-cation-exchange polymer and diaphorase. Chemistry Letters, (12), 1093-1094. https://doi.org/10.1246/cl.1996.1093

Preparative, electroenzymatic reduction of NAD⁺ to NADH on a thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen-cation-exchange polymer and diaphorase. / Kashiwagi, Yoshitomo; Yanagisawa, Yoshinori; Shibayama, Norihiko et al.
In: Chemistry Letters, No. 12, 1996, p. 1093-1094.

Research output: Contribution to journal › Article › peer-review

Kashiwagi, Y, Yanagisawa, Y, Shibayama, N, Nakahara, K, Kurashima, F, Anzai, JI & Osa, T 1996, 'Preparative, electroenzymatic reduction of NAD⁺ to NADH on a thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen-cation-exchange polymer and diaphorase', Chemistry Letters, no. 12, pp. 1093-1094. https://doi.org/10.1246/cl.1996.1093

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author = "Yoshitomo Kashiwagi and Yoshinori Yanagisawa and Norihiko Shibayama and Kaname Nakahara and Futoshi Kurashima and Anzai, {Jun Ichi} and Tetsuo Osa",

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doi = "10.1246/cl.1996.1093",

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AU - Kashiwagi, Yoshitomo

AU - Yanagisawa, Yoshinori

AU - Shibayama, Norihiko

AU - Nakahara, Kaname

AU - Kurashima, Futoshi

AU - Anzai, Jun Ichi

AU - Osa, Tetsuo

PY - 1996

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Preparative, electroenzymatic reduction of NAD⁺ to NADH on a thin poly(acrylic acid) layer-coated graphite felt electrode coimmobilizing ion-paired methyl viologen-cation-exchange polymer and diaphorase

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