TY - JOUR
T1 - Prepartion and antigenic properties of estriol 16-glucuronide- and estradiol 17-glucuronide-[C-6]-bovine serum albumin conjugates
AU - Nambara, Toshio
AU - Shimada, Kazutake
AU - Ohkubo, Tadashi
AU - Niwa, Toshifumi
PY - 1982/4
Y1 - 1982/4
N2 - The preparation and antigenic properties of estriol 16-glucuronide-bovine serum albumin (BSA) and estradiol 17-glucuronide-BSA conjugates in which the hapten molecule is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed excellent specificity to estriol 16-glucuronide and estradiol 17-glucuronide, respectively, exhibiting no significant cross-reactions with estrogen glucuronides having a substituent in ring A and no cross-reactivities with free estrogens. their sulfates and related steroids.
AB - The preparation and antigenic properties of estriol 16-glucuronide-bovine serum albumin (BSA) and estradiol 17-glucuronide-BSA conjugates in which the hapten molecule is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed excellent specificity to estriol 16-glucuronide and estradiol 17-glucuronide, respectively, exhibiting no significant cross-reactions with estrogen glucuronides having a substituent in ring A and no cross-reactivities with free estrogens. their sulfates and related steroids.
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U2 - 10.1016/0022-4731(82)90075-9
DO - 10.1016/0022-4731(82)90075-9
M3 - Article
C2 - 7087478
AN - SCOPUS:0019995857
SN - 0960-0760
VL - 16
SP - 533
EP - 538
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 4
ER -