Presence of immunoreactive endothelin in human milk

Hing Chung Lam; Kazuhiro Takahashi; Mohammed A. Ghatei; Stephen R. Bloom

doi:10.1016/0014-5793(90)80666-7

Presence of immunoreactive endothelin in human milk

Hing Chung Lam, Kazuhiro Takahashi, Mohammed A. Ghatei, Stephen R. Bloom

MED - Health Sciences

Hammersmith Hospital

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 ± 1.6 pmol l(mean ± SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.

Original language	English
Pages (from-to)	184-186
Number of pages	3
Journal	FEBS Letters
Volume	261
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80666-7
Publication status	Published - 1990 Feb 12

Keywords

Chromatography
Endothelin
Human milk
Radioimmunoassay

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10.1016/0014-5793(90)80666-7

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abstract = "Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 ± 1.6 pmol l(mean ± SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.",

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AU - Lam, Hing Chung

AU - Takahashi, Kazuhiro

AU - Ghatei, Mohammed A.

AU - Bloom, Stephen R.

PY - 1990/2/12

Y1 - 1990/2/12

N2 - Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 ± 1.6 pmol l(mean ± SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.

AB - Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 ± 1.6 pmol l(mean ± SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.

KW - Chromatography

KW - Endothelin

KW - Human milk

KW - Radioimmunoassay

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Presence of immunoreactive endothelin in human milk

Abstract

Keywords

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