Presence of phosphatidylcholine hydroperoxide in human plasma

Teruo Miyazawa; Keiichi Yasuda; Kenshiro Fujimoto; Takashi Kaneda

doi:10.1093/oxfordjournals.jbchem.a122339

Presence of phosphatidylcholine hydroperoxide in human plasma

Teruo Miyazawa, Keiichi Yasuda, Kenshiro Fujimoto, Takashi Kaneda

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article › peer-review

107 Citations (Scopus)

Abstract

A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cyto-chrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O₂. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.

Original language	English
Pages (from-to)	744-746
Number of pages	3
Journal	Journal of biochemistry
Volume	103
Issue number	5
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a122339
Publication status	Published - 1988 May

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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title = "Presence of phosphatidylcholine hydroperoxide in human plasma",

abstract = "A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cyto-chrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.",

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AU - Miyazawa, Teruo

AU - Yasuda, Keiichi

AU - Fujimoto, Kenshiro

AU - Kaneda, Takashi

PY - 1988/5

Y1 - 1988/5

N2 - A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cyto-chrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.

AB - A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cyto-chrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.

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Presence of phosphatidylcholine hydroperoxide in human plasma

Abstract

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