Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam

Sam Thi Nguyen; Yasuhiro Fukuda; Duc Tan Nguyen; Chika Tada; Yutaka Nakai

doi:10.1007/s11250-016-1013-x

Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam

Sam Thi Nguyen, Yasuhiro Fukuda, Duc Tan Nguyen, Chika Tada, Yutaka Nakai

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Little information is available on the epidemiology of Giardia duodenalis in beef cattle from Vietnam. This study was performed to determine the prevalence and genotypes/assemblages of G. duodenalis in native beef calves younger than 6 months in the region. A total of 412 calf fecal samples, randomly selected from 99 small-scale farms located in DacLac and KhanhHoa provinces, central Vietnam, were screened for the presence of G. duodenalis cysts using the zinc-sulfate flotation method followed by iodine staining. The overall prevalence on the sample and herd levels were 13.8 % (57/412) and 42.4 % (42/99), respectively. Molecular analysis in the β-giardin and triosephosphate isomerase genes demonstrated the presence of only G. duodenalis assemblage E in the animals. Since assemblage E has been rarely reported in humans, the zoonotic risk in beef calves in the region appears to be minimal.

Original language	English
Pages (from-to)	837-841
Number of pages	5
Journal	Tropical Animal Health and Production
Volume	48
Issue number	4
DOIs	https://doi.org/10.1007/s11250-016-1013-x
Publication status	Published - 2016 Apr 1

Keywords

Calves
Genotyping
Giardia duodenalis
Prevalence
Vietnam

Access to Document

10.1007/s11250-016-1013-x

Cite this

@article{619d03c7385640edb3512c317e314993,

title = "Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam",

abstract = "Little information is available on the epidemiology of Giardia duodenalis in beef cattle from Vietnam. This study was performed to determine the prevalence and genotypes/assemblages of G. duodenalis in native beef calves younger than 6 months in the region. A total of 412 calf fecal samples, randomly selected from 99 small-scale farms located in DacLac and KhanhHoa provinces, central Vietnam, were screened for the presence of G. duodenalis cysts using the zinc-sulfate flotation method followed by iodine staining. The overall prevalence on the sample and herd levels were 13.8 % (57/412) and 42.4 % (42/99), respectively. Molecular analysis in the β-giardin and triosephosphate isomerase genes demonstrated the presence of only G. duodenalis assemblage E in the animals. Since assemblage E has been rarely reported in humans, the zoonotic risk in beef calves in the region appears to be minimal.",

keywords = "Calves, Genotyping, Giardia duodenalis, Prevalence, Vietnam",

author = "Nguyen, {Sam Thi} and Yasuhiro Fukuda and Nguyen, {Duc Tan} and Chika Tada and Yutaka Nakai",

note = "Funding Information: This study was financially supported by the National Foundation of Science and Technology Development (NAFOSTED), Ministry of Science and Technology of Vietnam, under a Research Grant coded 106-NN.05-2014.10. The authors acknowledge Drs. Le Duc Quyet and Nguyen Van Thoai for their technical supports during the study. Publisher Copyright: {\textcopyright} 2016, Springer Science+Business Media Dordrecht.",

year = "2016",

month = apr,

day = "1",

doi = "10.1007/s11250-016-1013-x",

language = "English",

volume = "48",

pages = "837--841",

journal = "Tropical Animal Health and Production",

issn = "0049-4747",

publisher = "Springer Netherlands",

number = "4",

}

TY - JOUR

T1 - Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam

AU - Nguyen, Sam Thi

AU - Fukuda, Yasuhiro

AU - Nguyen, Duc Tan

AU - Tada, Chika

AU - Nakai, Yutaka

N1 - Funding Information: This study was financially supported by the National Foundation of Science and Technology Development (NAFOSTED), Ministry of Science and Technology of Vietnam, under a Research Grant coded 106-NN.05-2014.10. The authors acknowledge Drs. Le Duc Quyet and Nguyen Van Thoai for their technical supports during the study. Publisher Copyright: © 2016, Springer Science+Business Media Dordrecht.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Little information is available on the epidemiology of Giardia duodenalis in beef cattle from Vietnam. This study was performed to determine the prevalence and genotypes/assemblages of G. duodenalis in native beef calves younger than 6 months in the region. A total of 412 calf fecal samples, randomly selected from 99 small-scale farms located in DacLac and KhanhHoa provinces, central Vietnam, were screened for the presence of G. duodenalis cysts using the zinc-sulfate flotation method followed by iodine staining. The overall prevalence on the sample and herd levels were 13.8 % (57/412) and 42.4 % (42/99), respectively. Molecular analysis in the β-giardin and triosephosphate isomerase genes demonstrated the presence of only G. duodenalis assemblage E in the animals. Since assemblage E has been rarely reported in humans, the zoonotic risk in beef calves in the region appears to be minimal.

AB - Little information is available on the epidemiology of Giardia duodenalis in beef cattle from Vietnam. This study was performed to determine the prevalence and genotypes/assemblages of G. duodenalis in native beef calves younger than 6 months in the region. A total of 412 calf fecal samples, randomly selected from 99 small-scale farms located in DacLac and KhanhHoa provinces, central Vietnam, were screened for the presence of G. duodenalis cysts using the zinc-sulfate flotation method followed by iodine staining. The overall prevalence on the sample and herd levels were 13.8 % (57/412) and 42.4 % (42/99), respectively. Molecular analysis in the β-giardin and triosephosphate isomerase genes demonstrated the presence of only G. duodenalis assemblage E in the animals. Since assemblage E has been rarely reported in humans, the zoonotic risk in beef calves in the region appears to be minimal.

KW - Calves

KW - Genotyping

KW - Giardia duodenalis

KW - Prevalence

KW - Vietnam

UR - http://www.scopus.com/inward/record.url?scp=84961875527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961875527&partnerID=8YFLogxK

U2 - 10.1007/s11250-016-1013-x

DO - 10.1007/s11250-016-1013-x

M3 - Article

C2 - 26922741

AN - SCOPUS:84961875527

SN - 0049-4747

VL - 48

SP - 837

EP - 841

JO - Tropical Animal Health and Production

JF - Tropical Animal Health and Production

IS - 4

ER -

Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this