Prevention of initial supercooling in progressive freeze-concentration

Ling Liu, Tomoyuki Fujii, Kiro Hayakawa, Osato Miyawaki

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


A physical method is proposed that uses a cooling plate with many small holes to prevent initial supercooling in progressive freeze-concentration, and thus avoid serious contamination of the ice produced. The higher chance for ice nucleation of the water molecules in the holes due to the temperature gradient in the cooling plate resulted in the initial supercooling being completely prevented. Accordingly, the purity of the ice initially formed was substantially improved when compared with that by the standard vessel without holes in the cooling plate.

Original languageEnglish
Pages (from-to)2467-2469
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number12
Publication statusPublished - 1998
Externally publishedYes


  • Freeze concentration
  • Progressive freezing
  • Purity of ice
  • Supercooling

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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