Primary structure and distribution of a novel ryanodine receptor/calcium release channel from rabbit brain

Yasuhiro Hakamata; Junichi Nakai; Hiroshi Takeshima; Keiji Imoto

doi:10.1016/0014-5793(92)80941-9

Primary structure and distribution of a novel ryanodine receptor/calcium release channel from rabbit brain

Yasuhiro Hakamata, Junichi Nakai, Hiroshi Takeshima, Keiji Imoto

DEN - Dental Sciences

Kyoto University

Research output: Contribution to journal › Article › peer-review

379 Citations (Scopus)

Abstract

The complete amino acid sequence of a novel ryanodine receptor/calcium release channel from rabbit brain has been deduced by cloning and sequence analysis of the EDNA. This protein is composed of 4872 amino acids and shares characteristic structural features with the skeletal muscle and cardiac ryanodine receptors. RNA blot hybridization analysis shows that the brain ryanodine receptor is abundantly expressed in corpus striatum, thalamus and hippocampus, whereas the cardiac ryanodine receptor is more uniformly expressed in the brain. The brain ryanodine receptor gene is transcribed also in smooth muscle.

Original language	English
Pages (from-to)	229-235
Number of pages	7
Journal	FEBS Letters
Volume	312
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(92)80941-9
Publication status	Published - 1992 Nov 9

Keywords

Calcium release channel
cDNA cloning
Rabbit brain
RNA blot hybridization
Ryanodine receptor
Smooth muscle

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10.1016/0014-5793(92)80941-9

Cite this

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title = "Primary structure and distribution of a novel ryanodine receptor/calcium release channel from rabbit brain",

abstract = "The complete amino acid sequence of a novel ryanodine receptor/calcium release channel from rabbit brain has been deduced by cloning and sequence analysis of the EDNA. This protein is composed of 4872 amino acids and shares characteristic structural features with the skeletal muscle and cardiac ryanodine receptors. RNA blot hybridization analysis shows that the brain ryanodine receptor is abundantly expressed in corpus striatum, thalamus and hippocampus, whereas the cardiac ryanodine receptor is more uniformly expressed in the brain. The brain ryanodine receptor gene is transcribed also in smooth muscle.",

keywords = "Calcium release channel, cDNA cloning, Rabbit brain, RNA blot hybridization, Ryanodine receptor, Smooth muscle",

author = "Yasuhiro Hakamata and Junichi Nakai and Hiroshi Takeshima and Keiji Imoto",

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AU - Hakamata, Yasuhiro

AU - Nakai, Junichi

AU - Takeshima, Hiroshi

AU - Imoto, Keiji

N1 - Funding Information: Acknowledgements: We thank the late Professor Shosaku Numa for his support and encouragement and Dr. Takashi Miyata and Mr. Naoyuki lwabe for the computer analysis. This investigation was supported in part by research grants from the Ministry of Education, Science and Culture of Japan and the Institute of Physical and Chemical Re~eareh.

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N2 - The complete amino acid sequence of a novel ryanodine receptor/calcium release channel from rabbit brain has been deduced by cloning and sequence analysis of the EDNA. This protein is composed of 4872 amino acids and shares characteristic structural features with the skeletal muscle and cardiac ryanodine receptors. RNA blot hybridization analysis shows that the brain ryanodine receptor is abundantly expressed in corpus striatum, thalamus and hippocampus, whereas the cardiac ryanodine receptor is more uniformly expressed in the brain. The brain ryanodine receptor gene is transcribed also in smooth muscle.

AB - The complete amino acid sequence of a novel ryanodine receptor/calcium release channel from rabbit brain has been deduced by cloning and sequence analysis of the EDNA. This protein is composed of 4872 amino acids and shares characteristic structural features with the skeletal muscle and cardiac ryanodine receptors. RNA blot hybridization analysis shows that the brain ryanodine receptor is abundantly expressed in corpus striatum, thalamus and hippocampus, whereas the cardiac ryanodine receptor is more uniformly expressed in the brain. The brain ryanodine receptor gene is transcribed also in smooth muscle.

KW - Calcium release channel

KW - cDNA cloning

KW - Rabbit brain

KW - RNA blot hybridization

KW - Ryanodine receptor

KW - Smooth muscle

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Primary structure and distribution of a novel ryanodine receptor/calcium release channel from rabbit brain

Abstract

Keywords

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