TY - JOUR
T1 - Primary structure and functional expression of the ω-conotoxin-sensitive N-type calcium channel from rabbit brain
AU - Fujita, Yoshihiko
AU - Mynlieff, Michelle
AU - Dirksen, Robert T.
AU - Kim, Man Suk
AU - Niidome, Tetsuhiro
AU - Nakai, Junichi
AU - Friedrich, Thomas
AU - Iwabe, Naoyuki
AU - Miyata, Takashi
AU - Furuichi, Teiichi
AU - Furutama, Daisuke
AU - Mikoshiba, Katsuhiko
AU - Mori, Yasuo
AU - Beam, Kurt G.
N1 - Funding Information:
We thank the late professor Shosaku Numa for his encouragement and support for this project. We also thank Keiji lmoto and Haruyuki Atomi for critically reading the manuscript, and Miwa Kuchiishi, Lorrie Bennett, Aaron Beam, and Jennifer Han-neman for excellent technical assistance. This investigation was supported by the Ministry of Education, Science and Culture of Japan, the Japanese Foundation of Metabolism and Diseases, the Institute of Physical and Chemical Research (RIKEN) (to Y. M.), and National Institutes of Health grants NS-24444 and NS-26416 (to K. G. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact.
PY - 1993/4
Y1 - 1993/4
N2 - The complete amino acid sequence of a rabbit brain calcium channel (BIII) has been deduced by cloning and sequencing cDNA. The open reading frame encodes 2339 amino acids, which corresponds to an Mr of 261,167. A phylogenetic tree representing evolutionary relationships indicates that BIII is grouped together with the other rabbit brain calcium channels, BI and BII, into a subfamily t hat is distinct from the dihydropyridine-sensitive L-type subfamily. Transient expression in cultured skeletal muscle myotubes derived from muscular dysgenic mice demonstrates that the BIII channel mediates an ω-conotoxin-sensitive calcium current with kinetics and voltage dependence like those previously reported for whole-cell N-type current. Cell-attached patch recordings, with isotonic barium as the charge carrier, revealed distinct single channels with an average slope conductance of 14.3 pS.
AB - The complete amino acid sequence of a rabbit brain calcium channel (BIII) has been deduced by cloning and sequencing cDNA. The open reading frame encodes 2339 amino acids, which corresponds to an Mr of 261,167. A phylogenetic tree representing evolutionary relationships indicates that BIII is grouped together with the other rabbit brain calcium channels, BI and BII, into a subfamily t hat is distinct from the dihydropyridine-sensitive L-type subfamily. Transient expression in cultured skeletal muscle myotubes derived from muscular dysgenic mice demonstrates that the BIII channel mediates an ω-conotoxin-sensitive calcium current with kinetics and voltage dependence like those previously reported for whole-cell N-type current. Cell-attached patch recordings, with isotonic barium as the charge carrier, revealed distinct single channels with an average slope conductance of 14.3 pS.
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U2 - 10.1016/0896-6273(93)90162-K
DO - 10.1016/0896-6273(93)90162-K
M3 - Article
C2 - 8386525
AN - SCOPUS:0027316231
SN - 0896-6273
VL - 10
SP - 585
EP - 598
JO - Neuron
JF - Neuron
IS - 4
ER -