Probing biological systems with near-field optics

Hitoshi Shiku; Christopher W. Hollars; M. Annie Lee; Chad E. Talley; Greg Cooksey; Robert C. Dunn

doi:10.1117/12.306124

Probing biological systems with near-field optics

Hitoshi Shiku, Christopher W. Hollars, M. Annie Lee, Chad E. Talley, Greg Cooksey, Robert C. Dunn

Research output: Contribution to journal › Conference article › peer-review

1 Citation (Scopus)

Abstract

The imaging characteristics of cantilevered NSOM probes operating in a tapping-mode feedback arrangement are discussed and compared to conventional tips employing the shear-force feedback method. Images from a wide range of samples are presented to demonstrate the surface tracking capabilities over both high and low topology samples, in addition to the low fluorescence detection limits possible utilizing the new tips. The results show that the cantilevered tip operating in a tapping-mode arrangement offers enhanced force imaging of the sample topology without compromising the low detection limits or high spatial resolution of the NSOM fluorescence images. The examples discussed here indicate that the new design will be particularly useful for applications involving biological samples that frequently exhibit complex surface topologies.

Original language	English
Pages (from-to)	156-164
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3273
DOIs	https://doi.org/10.1117/12.306124
Publication status	Published - 1998 Dec 1
Externally published	Yes
Event	Laser Techniques for Condensed-Phase and Biological Systems - San Jose, CA, United States Duration: 1998 Jan 29 → 1998 Jan 31

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.306124

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title = "Probing biological systems with near-field optics",

abstract = "The imaging characteristics of cantilevered NSOM probes operating in a tapping-mode feedback arrangement are discussed and compared to conventional tips employing the shear-force feedback method. Images from a wide range of samples are presented to demonstrate the surface tracking capabilities over both high and low topology samples, in addition to the low fluorescence detection limits possible utilizing the new tips. The results show that the cantilevered tip operating in a tapping-mode arrangement offers enhanced force imaging of the sample topology without compromising the low detection limits or high spatial resolution of the NSOM fluorescence images. The examples discussed here indicate that the new design will be particularly useful for applications involving biological samples that frequently exhibit complex surface topologies.",

author = "Hitoshi Shiku and Hollars, {Christopher W.} and Lee, {M. Annie} and Talley, {Chad E.} and Greg Cooksey and Dunn, {Robert C.}",

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doi = "10.1117/12.306124",

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note = "Laser Techniques for Condensed-Phase and Biological Systems ; Conference date: 29-01-1998 Through 31-01-1998",

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TY - JOUR

T1 - Probing biological systems with near-field optics

AU - Shiku, Hitoshi

AU - Hollars, Christopher W.

AU - Lee, M. Annie

AU - Talley, Chad E.

AU - Cooksey, Greg

AU - Dunn, Robert C.

PY - 1998/12/1

Y1 - 1998/12/1

N2 - The imaging characteristics of cantilevered NSOM probes operating in a tapping-mode feedback arrangement are discussed and compared to conventional tips employing the shear-force feedback method. Images from a wide range of samples are presented to demonstrate the surface tracking capabilities over both high and low topology samples, in addition to the low fluorescence detection limits possible utilizing the new tips. The results show that the cantilevered tip operating in a tapping-mode arrangement offers enhanced force imaging of the sample topology without compromising the low detection limits or high spatial resolution of the NSOM fluorescence images. The examples discussed here indicate that the new design will be particularly useful for applications involving biological samples that frequently exhibit complex surface topologies.

AB - The imaging characteristics of cantilevered NSOM probes operating in a tapping-mode feedback arrangement are discussed and compared to conventional tips employing the shear-force feedback method. Images from a wide range of samples are presented to demonstrate the surface tracking capabilities over both high and low topology samples, in addition to the low fluorescence detection limits possible utilizing the new tips. The results show that the cantilevered tip operating in a tapping-mode arrangement offers enhanced force imaging of the sample topology without compromising the low detection limits or high spatial resolution of the NSOM fluorescence images. The examples discussed here indicate that the new design will be particularly useful for applications involving biological samples that frequently exhibit complex surface topologies.

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DO - 10.1117/12.306124

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EP - 164

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Laser Techniques for Condensed-Phase and Biological Systems

Y2 - 29 January 1998 through 31 January 1998

ER -

Probing biological systems with near-field optics

Abstract

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