Probing biological systems with near-field optics

Hitoshi Shiku, Christopher W. Hollars, M. Annie Lee, Chad E. Talley, Greg Cooksey, Robert C. Dunn

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)


The imaging characteristics of cantilevered NSOM probes operating in a tapping-mode feedback arrangement are discussed and compared to conventional tips employing the shear-force feedback method. Images from a wide range of samples are presented to demonstrate the surface tracking capabilities over both high and low topology samples, in addition to the low fluorescence detection limits possible utilizing the new tips. The results show that the cantilevered tip operating in a tapping-mode arrangement offers enhanced force imaging of the sample topology without compromising the low detection limits or high spatial resolution of the NSOM fluorescence images. The examples discussed here indicate that the new design will be particularly useful for applications involving biological samples that frequently exhibit complex surface topologies.

Original languageEnglish
Pages (from-to)156-164
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Publication statusPublished - 1998 Dec 1
Externally publishedYes
EventLaser Techniques for Condensed-Phase and Biological Systems - San Jose, CA, United States
Duration: 1998 Jan 291998 Jan 31

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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