Production and secretion of adrenomedullin in cultured human alveolar macrophages

Masaharu Nakayama; Kazuhiro Takahashi; Osamu Murakami; Masaru Yanai; Hidetada Sasaki; Kunio Shirato; Shigeki Shibahara

doi:10.1016/S0196-9781(99)00107-2

Production and secretion of adrenomedullin in cultured human alveolar macrophages

Masaharu Nakayama, Kazuhiro Takahashi, Osamu Murakami, Masaru Yanai, Hidetada Sasaki, Kunio Shirato, Shigeki Shibahara

Tohoku University

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/10⁵ cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

Original language	English
Pages (from-to)	1123-1125
Number of pages	3
Journal	Peptides
Volume	20
Issue number	9
DOIs	https://doi.org/10.1016/S0196-9781(99)00107-2
Publication status	Published - 1999 Sept

Keywords

Adrenomedullin
Alveolar macrophages

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10.1016/S0196-9781(99)00107-2

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title = "Production and secretion of adrenomedullin in cultured human alveolar macrophages",

abstract = "To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.",

keywords = "Adrenomedullin, Alveolar macrophages",

author = "Masaharu Nakayama and Kazuhiro Takahashi and Osamu Murakami and Masaru Yanai and Hidetada Sasaki and Kunio Shirato and Shigeki Shibahara",

note = "Funding Information: This work has been supported partly by Grants-in-aid for Scientific Research (B and C) and for Exploratory Research from the Ministry of Education, Science, Sports and Culture of Japan, and by the Gonryou Medical Foundation.",

year = "1999",

month = sep,

doi = "10.1016/S0196-9781(99)00107-2",

language = "English",

volume = "20",

pages = "1123--1125",

journal = "Peptides",

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TY - JOUR

T1 - Production and secretion of adrenomedullin in cultured human alveolar macrophages

AU - Nakayama, Masaharu

AU - Takahashi, Kazuhiro

AU - Murakami, Osamu

AU - Yanai, Masaru

AU - Sasaki, Hidetada

AU - Shirato, Kunio

AU - Shibahara, Shigeki

N1 - Funding Information: This work has been supported partly by Grants-in-aid for Scientific Research (B and C) and for Exploratory Research from the Ministry of Education, Science, Sports and Culture of Japan, and by the Gonryou Medical Foundation.

PY - 1999/9

Y1 - 1999/9

N2 - To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

AB - To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

KW - Adrenomedullin

KW - Alveolar macrophages

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AN - SCOPUS:0032878111

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VL - 20

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JO - Peptides

JF - Peptides

IS - 9

ER -

Production and secretion of adrenomedullin in cultured human alveolar macrophages

Abstract

Keywords

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