Production and secretion of adrenomedullin in cultured human alveolar macrophages

Masaharu Nakayama, Kazuhiro Takahashi, Osamu Murakami, Masaru Yanai, Hidetada Sasaki, Kunio Shirato, Shigeki Shibahara

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)1123-1125
Number of pages3
Issue number9
Publication statusPublished - 1999 Sept


  • Adrenomedullin
  • Alveolar macrophages


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