Production of term offspring by in vitro fertilization using old mouse spermatozoa

Teruhiko Wakayama; Kentaro Tanemura; Masamichi Kurohmaru; Yoshihiro Hayashi; Junichi Suto; Kenkichi Imamura; Katsuhiro Fukuta; Hidenori Mori; Kazunao Kuramoto

doi:10.1292/jvms.57.545

Production of term offspring by in vitro fertilization using old mouse spermatozoa

Teruhiko Wakayama, Kentaro Tanemura, Masamichi Kurohmaru, Yoshihiro Hayashi, Junichi Suto, Kenkichi Imamura, Katsuhiro Fukuta, Hidenori Mori, Kazunao Kuramoto

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We successfully produced offspring of old male BDF₁ mice using in vitro fertilization. Although 7 old male mice (33 months of age) were infertile and revealed frequent degeneration in the seminiferous epithelia, 4 of them had spermatozoa in the caudae epididymides. The IVF rate of their sperm with eggs from young ICR mice was very high (82.0%). The production rate of offspring was also satisfactory (61.6%). The present study indicates that the IVF method is useful for producing offspring of aged male mice.

Original language	English
Pages (from-to)	545-547
Number of pages	3
Journal	Journal of Veterinary Medical Science
Volume	57
Issue number	3
DOIs	https://doi.org/10.1292/jvms.57.545
Publication status	Published - 1995

Keywords

aging
fertilization
spermatozoa

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10.1292/jvms.57.545

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title = "Production of term offspring by in vitro fertilization using old mouse spermatozoa",

abstract = "We successfully produced offspring of old male BDF1 mice using in vitro fertilization. Although 7 old male mice (33 months of age) were infertile and revealed frequent degeneration in the seminiferous epithelia, 4 of them had spermatozoa in the caudae epididymides. The IVF rate of their sperm with eggs from young ICR mice was very high (82.0%). The production rate of offspring was also satisfactory (61.6%). The present study indicates that the IVF method is useful for producing offspring of aged male mice.",

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author = "Teruhiko Wakayama and Kentaro Tanemura and Masamichi Kurohmaru and Yoshihiro Hayashi and Junichi Suto and Kenkichi Imamura and Katsuhiro Fukuta and Hidenori Mori and Kazunao Kuramoto",

year = "1995",

doi = "10.1292/jvms.57.545",

language = "English",

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T1 - Production of term offspring by in vitro fertilization using old mouse spermatozoa

AU - Wakayama, Teruhiko

AU - Tanemura, Kentaro

AU - Kurohmaru, Masamichi

AU - Hayashi, Yoshihiro

AU - Suto, Junichi

AU - Imamura, Kenkichi

AU - Fukuta, Katsuhiro

AU - Mori, Hidenori

AU - Kuramoto, Kazunao

PY - 1995

Y1 - 1995

N2 - We successfully produced offspring of old male BDF1 mice using in vitro fertilization. Although 7 old male mice (33 months of age) were infertile and revealed frequent degeneration in the seminiferous epithelia, 4 of them had spermatozoa in the caudae epididymides. The IVF rate of their sperm with eggs from young ICR mice was very high (82.0%). The production rate of offspring was also satisfactory (61.6%). The present study indicates that the IVF method is useful for producing offspring of aged male mice.

AB - We successfully produced offspring of old male BDF1 mice using in vitro fertilization. Although 7 old male mice (33 months of age) were infertile and revealed frequent degeneration in the seminiferous epithelia, 4 of them had spermatozoa in the caudae epididymides. The IVF rate of their sperm with eggs from young ICR mice was very high (82.0%). The production rate of offspring was also satisfactory (61.6%). The present study indicates that the IVF method is useful for producing offspring of aged male mice.

KW - aging

KW - fertilization

KW - spermatozoa

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JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

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ER -

Production of term offspring by in vitro fertilization using old mouse spermatozoa

Abstract

Keywords

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