Production of term offspring by in vitro fertilization using old mouse spermatozoa

Teruhiko Wakayama, Kentaro Tanemura, Masamichi Kurohmaru, Yoshihiro Hayashi, Junichi Suto, Kenkichi Imamura, Katsuhiro Fukuta, Hidenori Mori, Kazunao Kuramoto

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


We successfully produced offspring of old male BDF1 mice using in vitro fertilization. Although 7 old male mice (33 months of age) were infertile and revealed frequent degeneration in the seminiferous epithelia, 4 of them had spermatozoa in the caudae epididymides. The IVF rate of their sperm with eggs from young ICR mice was very high (82.0%). The production rate of offspring was also satisfactory (61.6%). The present study indicates that the IVF method is useful for producing offspring of aged male mice.

Original languageEnglish
Pages (from-to)545-547
Number of pages3
JournalJournal of Veterinary Medical Science
Issue number3
Publication statusPublished - 1995


  • aging
  • fertilization
  • spermatozoa


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