Production of truncated protein by the crosslink formation of mRNA with 2′-OMe oligoribonucleotide containing 2-amino-6-vinylpurine

Shinya Hagihara; Shuhei Kusano; Wei Chen Lin; Xiao Guang Chao; Tsuneaki Hori; Shuhei Imoto; Fumi Nagatsugi

doi:10.1016/j.bmcl.2012.04.123

Production of truncated protein by the crosslink formation of mRNA with 2′-OMe oligoribonucleotide containing 2-amino-6-vinylpurine

Shinya Hagihara, Shuhei Kusano, Wei Chen Lin, Xiao Guang Chao, Tsuneaki Hori, Shuhei Imoto, Fumi Nagatsugi

IMRAM - Division of Organic- and Biomaterials Research

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.

Original language	English
Pages (from-to)	3870-3872
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	22
Issue number	12
DOIs	https://doi.org/10.1016/j.bmcl.2012.04.123
Publication status	Published - 2012 Jun 15

Keywords

Antisense
Crosslink
Translational inhibition
Truncated proteins

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10.1016/j.bmcl.2012.04.123

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title = "Production of truncated protein by the crosslink formation of mRNA with 2′-OMe oligoribonucleotide containing 2-amino-6-vinylpurine",

abstract = "The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.",

keywords = "Antisense, Crosslink, Translational inhibition, Truncated proteins",

author = "Shinya Hagihara and Shuhei Kusano and Lin, {Wei Chen} and Chao, {Xiao Guang} and Tsuneaki Hori and Shuhei Imoto and Fumi Nagatsugi",

note = "Funding Information: We thank T. Chikuni for her contributions to the synthesis and evaluation of the CFOs. This work was supported by a KAKENHI (23750180) Grant-in-Aid for Young Scientists (B) and for Scientific Research (S) and (B), and CREST from Japan Science and Technology Agency. ",

year = "2012",

month = jun,

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doi = "10.1016/j.bmcl.2012.04.123",

language = "English",

volume = "22",

pages = "3870--3872",

journal = "Bioorganic and Medicinal Chemistry Letters",

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publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Production of truncated protein by the crosslink formation of mRNA with 2′-OMe oligoribonucleotide containing 2-amino-6-vinylpurine

AU - Hagihara, Shinya

AU - Kusano, Shuhei

AU - Lin, Wei Chen

AU - Chao, Xiao Guang

AU - Hori, Tsuneaki

AU - Imoto, Shuhei

AU - Nagatsugi, Fumi

N1 - Funding Information: We thank T. Chikuni for her contributions to the synthesis and evaluation of the CFOs. This work was supported by a KAKENHI (23750180) Grant-in-Aid for Young Scientists (B) and for Scientific Research (S) and (B), and CREST from Japan Science and Technology Agency.

PY - 2012/6/15

Y1 - 2012/6/15

N2 - The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.

AB - The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.

KW - Antisense

KW - Crosslink

KW - Translational inhibition

KW - Truncated proteins

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SN - 0960-894X

VL - 22

SP - 3870

EP - 3872

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 12

ER -

Production of truncated protein by the crosslink formation of mRNA with 2′-OMe oligoribonucleotide containing 2-amino-6-vinylpurine

Abstract

Keywords

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