TY - JOUR
T1 - Profiles of DARPP-32 in the insular cortex with schizophrenia
T2 - A postmortem brain study
AU - Nishiura, Keisuke
AU - Kunii, Yasuto
AU - Wada, Akira
AU - Matsumoto, Junya
AU - Yang, Qiaohui
AU - Ikemoto, Keiko
AU - Niwa, Shin Ichi
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (B) (grant number: 20390315 ) from the Japan Society for the Promotion of Science (JSPS) .
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - In patients with schizophrenia, various physical disorders are sometimes discovered only when they have reached a later and more severe stage. This phenomenon is believed to be caused, at least in part, by an increase in pain threshold. The gamma-aminobutyric acid (GABA)-ergic and glutamatergic systems in the rostral agranular insular cortex (RAIC) are thought to be involved in the regulation of pain threshold. However, no postmortem studies of the cerebral cortex have previously been published. Dopamine and cAMP-regulated phosphoprotein 32. kD (DARPP-32), which is involved in the GABAergic and glutamatergic systems, is considered to be crucial for elucidating the pathogenesis of schizophrenia.Using specific antibodies, we conducted immunohistochemical examinations of the RAIC in 10 subjects from a healthy control group, and 11 subjects from a schizophrenia group. The sex, age, and postmortem interval (PMI) of the schizophrenia group were matched to those of the healthy control group. We revealed that the density of DARPP-32-immunoreactive (IR) neurons in the II and III layers of the RAIC was significantly decreased (p < 0.05) in the schizophrenia group compared with the healthy control group. Our findings could partially explain the molecular basis of the pain threshold abnormalities found in patients with schizophrenia.
AB - In patients with schizophrenia, various physical disorders are sometimes discovered only when they have reached a later and more severe stage. This phenomenon is believed to be caused, at least in part, by an increase in pain threshold. The gamma-aminobutyric acid (GABA)-ergic and glutamatergic systems in the rostral agranular insular cortex (RAIC) are thought to be involved in the regulation of pain threshold. However, no postmortem studies of the cerebral cortex have previously been published. Dopamine and cAMP-regulated phosphoprotein 32. kD (DARPP-32), which is involved in the GABAergic and glutamatergic systems, is considered to be crucial for elucidating the pathogenesis of schizophrenia.Using specific antibodies, we conducted immunohistochemical examinations of the RAIC in 10 subjects from a healthy control group, and 11 subjects from a schizophrenia group. The sex, age, and postmortem interval (PMI) of the schizophrenia group were matched to those of the healthy control group. We revealed that the density of DARPP-32-immunoreactive (IR) neurons in the II and III layers of the RAIC was significantly decreased (p < 0.05) in the schizophrenia group compared with the healthy control group. Our findings could partially explain the molecular basis of the pain threshold abnormalities found in patients with schizophrenia.
KW - DARPP-32
KW - Immunohistochemistry
KW - Postmortem brains
KW - Rostral agranular insular cortex (RAIC)
KW - Schizophrenia
UR - http://www.scopus.com/inward/record.url?scp=82455199187&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82455199187&partnerID=8YFLogxK
U2 - 10.1016/j.pnpbp.2011.07.010
DO - 10.1016/j.pnpbp.2011.07.010
M3 - Article
C2 - 21821092
AN - SCOPUS:82455199187
SN - 0278-5846
VL - 35
SP - 1901
EP - 1907
JO - Progress in Neuro-Psychopharmacology and Biological Psychiatry
JF - Progress in Neuro-Psychopharmacology and Biological Psychiatry
IS - 8
ER -