Proliferation assay on a silicon chip applicable for tumors extirpated from mammalians

Yu Suke Torisawa, Hitoshi Shiku, Shigenobu Kasai, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We describe a novel anticancer drug sensitivity assay on a silicon chip applicable for tumors extirpated from in vivo mammalians. Human promyelocytic leukemia (HL-60) cells were subcutaneously (s.c.) inoculated in SCID mice, then removed 31 days after the inoculation. The cells were embedded in a small volume (18 nL) of a collagen-gel matrix on a pyramid-shaped silicon microstructure for further cultivation. The respiration activity of the cells on the chip was measured by scanning electrochemical microscopy (SECM). The proliferation behavior was continuously monitored for 6 days. It seemed that the proliferation rate of the cells removed from the mice was lower than that cultured in a flask and conformed to that in mice. The effects of cisplatin (CDDP) and etoposide (VP-16) on the HL-60 cultured in vivo were in good agreement with those obtained by a conventional colorimetric assay. Our results suggest that the SECM-based assay is appropriate for biopsy specimens in a relatively short-time evaluation.

Original languageEnglish
Pages (from-to)302-308
Number of pages7
JournalInternational Journal of Cancer
Volume109
Issue number2
DOIs
Publication statusPublished - 2004 Mar 20

Keywords

  • 3-D culture
  • Anticancer drug sensitivity assay
  • Collagen gel
  • Micromachining
  • Scanning electrochemical microscopy

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