Properties of the electrogenic activity of bacteriorhodopsin

Shizuma Miyazaki; Makoto Matsumoto; Søren Bo Brier; Toshihiro Higaki; Takumi Yamada; Tetsuaki Okamoto; Hiroshi Ueno; Shoichi Toyabe; Eiro Muneyuki

doi:10.1007/s00249-012-0870-0

Properties of the electrogenic activity of bacteriorhodopsin

Shizuma Miyazaki, Makoto Matsumoto, Søren Bo Brier, Toshihiro Higaki, Takumi Yamada, Tetsuaki Okamoto, Hiroshi Ueno, Shoichi Toyabe, Eiro Muneyuki

ENG - Applied Physics

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, "Lumirror" (Muneyuki et al. in FEBS Lett 427:109-114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.

Original language	English
Pages (from-to)	257-265
Number of pages	9
Journal	European Biophysics Journal
Volume	42
Issue number	4
DOIs	https://doi.org/10.1007/s00249-012-0870-0
Publication status	Published - 2013 Apr

Keywords

Electromotive force
Equivalent circuit
pH dependence
Photoelectric current
Proton pump

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10.1007/s00249-012-0870-0

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title = "Properties of the electrogenic activity of bacteriorhodopsin",

abstract = "In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, {"}Lumirror{"} (Muneyuki et al. in FEBS Lett 427:109-114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.",

keywords = "Electromotive force, Equivalent circuit, pH dependence, Photoelectric current, Proton pump",

author = "Shizuma Miyazaki and Makoto Matsumoto and Brier, {S{\o}ren Bo} and Toshihiro Higaki and Takumi Yamada and Tetsuaki Okamoto and Hiroshi Ueno and Shoichi Toyabe and Eiro Muneyuki",

note = "Funding Information: Acknowledgments We thank Toray Industries Inc. for providing the useful material {\textquoteleft}{\textquoteleft}Lumirror{\textquoteright}{\textquoteright}. We thank Messrs Sukuna Kurokawa and Masaki Watanabe for their contribution to the very first stage of this study and Drs Jun Tamogami, Takashi Kikukawa, Seiji Miyauchi, and Naoki Kamo for helpful discussion. The research was financially supported by Chuo University Joint Research Grant and Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) 21570172 to E.M.",

year = "2013",

month = apr,

doi = "10.1007/s00249-012-0870-0",

language = "English",

volume = "42",

pages = "257--265",

journal = "European Biophysics Journal",

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T1 - Properties of the electrogenic activity of bacteriorhodopsin

AU - Miyazaki, Shizuma

AU - Matsumoto, Makoto

AU - Brier, Søren Bo

AU - Higaki, Toshihiro

AU - Yamada, Takumi

AU - Okamoto, Tetsuaki

AU - Ueno, Hiroshi

AU - Toyabe, Shoichi

AU - Muneyuki, Eiro

N1 - Funding Information: Acknowledgments We thank Toray Industries Inc. for providing the useful material ‘‘Lumirror’’. We thank Messrs Sukuna Kurokawa and Masaki Watanabe for their contribution to the very first stage of this study and Drs Jun Tamogami, Takashi Kikukawa, Seiji Miyauchi, and Naoki Kamo for helpful discussion. The research was financially supported by Chuo University Joint Research Grant and Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) 21570172 to E.M.

PY - 2013/4

Y1 - 2013/4

N2 - In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, "Lumirror" (Muneyuki et al. in FEBS Lett 427:109-114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.

AB - In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, "Lumirror" (Muneyuki et al. in FEBS Lett 427:109-114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.

KW - Electromotive force

KW - Equivalent circuit

KW - pH dependence

KW - Photoelectric current

KW - Proton pump

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Properties of the electrogenic activity of bacteriorhodopsin

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Keywords

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