Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor

Zhuqing Wang; Jinhua Li; Mitsuteru Kimura; Takahito Ono

doi:10.1109/NANO.2016.7751536

Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor

Zhuqing Wang, Jinhua Li, Mitsuteru Kimura, Takahito Ono

ENG - Mechanical Systems Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Protein addressing in packaged multi-channels is developed for calorimetric biosensors in this research. First, we use electrodeposition method to fabricate chitosan film as the substrate on the patterned electrode surface. Second, the fabricated chitosan film was functionalized with alkyne groups for click chemistry. Thirdly, the azide-tagged protein is immobilized on the electrode by electrical signal for enzyme immobilization. The results demonstrate that the electro-click chemistry method was capable of multi-enzyme immobilization in packaged multi-channel for calorimetry biosensor of healthcare application.

Original language	English
Title of host publication	16th International Conference on Nanotechnology - IEEE NANO 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	681-682
Number of pages	2
ISBN (Electronic)	9781509039142
DOIs	https://doi.org/10.1109/NANO.2016.7751536
Publication status	Published - 2016 Nov 21
Event	16th IEEE International Conference on Nanotechnology - IEEE NANO 2016 - Sendai, Japan Duration: 2016 Aug 22 → 2016 Aug 25

Publication series

Name	16th International Conference on Nanotechnology - IEEE NANO 2016

Conference

Conference	16th IEEE International Conference on Nanotechnology - IEEE NANO 2016
Country/Territory	Japan
City	Sendai
Period	16/8/22 → 16/8/25

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10.1109/NANO.2016.7751536

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Wang, Z., Li, J., Kimura, M., & Ono, T. (2016). Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor. In 16th International Conference on Nanotechnology - IEEE NANO 2016 (pp. 681-682). Article 7751536 (16th International Conference on Nanotechnology - IEEE NANO 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2016.7751536

@inproceedings{74f331b4625d498f8345232b5f42ccbe,

title = "Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor",

abstract = "Protein addressing in packaged multi-channels is developed for calorimetric biosensors in this research. First, we use electrodeposition method to fabricate chitosan film as the substrate on the patterned electrode surface. Second, the fabricated chitosan film was functionalized with alkyne groups for click chemistry. Thirdly, the azide-tagged protein is immobilized on the electrode by electrical signal for enzyme immobilization. The results demonstrate that the electro-click chemistry method was capable of multi-enzyme immobilization in packaged multi-channel for calorimetry biosensor of healthcare application.",

author = "Zhuqing Wang and Jinhua Li and Mitsuteru Kimura and Takahito Ono",

note = "Funding Information: Resrach supported by the Center of Innovation (COI) program of Japan Science and Technology Agency. Publisher Copyright: {\textcopyright} 2016 IEEE.; 16th IEEE International Conference on Nanotechnology - IEEE NANO 2016 ; Conference date: 22-08-2016 Through 25-08-2016",

year = "2016",

month = nov,

day = "21",

doi = "10.1109/NANO.2016.7751536",

language = "English",

series = "16th International Conference on Nanotechnology - IEEE NANO 2016",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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address = "United States",

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Wang, Z, Li, J, Kimura, M & Ono, T 2016, Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor. in 16th International Conference on Nanotechnology - IEEE NANO 2016., 7751536, 16th International Conference on Nanotechnology - IEEE NANO 2016, Institute of Electrical and Electronics Engineers Inc., pp. 681-682, 16th IEEE International Conference on Nanotechnology - IEEE NANO 2016, Sendai, Japan, 16/8/22. https://doi.org/10.1109/NANO.2016.7751536

Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor. / Wang, Zhuqing; Li, Jinhua; Kimura, Mitsuteru et al.
16th International Conference on Nanotechnology - IEEE NANO 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 681-682 7751536 (16th International Conference on Nanotechnology - IEEE NANO 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor

AU - Wang, Zhuqing

AU - Li, Jinhua

AU - Kimura, Mitsuteru

AU - Ono, Takahito

PY - 2016/11/21

Y1 - 2016/11/21

N2 - Protein addressing in packaged multi-channels is developed for calorimetric biosensors in this research. First, we use electrodeposition method to fabricate chitosan film as the substrate on the patterned electrode surface. Second, the fabricated chitosan film was functionalized with alkyne groups for click chemistry. Thirdly, the azide-tagged protein is immobilized on the electrode by electrical signal for enzyme immobilization. The results demonstrate that the electro-click chemistry method was capable of multi-enzyme immobilization in packaged multi-channel for calorimetry biosensor of healthcare application.

AB - Protein addressing in packaged multi-channels is developed for calorimetric biosensors in this research. First, we use electrodeposition method to fabricate chitosan film as the substrate on the patterned electrode surface. Second, the fabricated chitosan film was functionalized with alkyne groups for click chemistry. Thirdly, the azide-tagged protein is immobilized on the electrode by electrical signal for enzyme immobilization. The results demonstrate that the electro-click chemistry method was capable of multi-enzyme immobilization in packaged multi-channel for calorimetry biosensor of healthcare application.

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U2 - 10.1109/NANO.2016.7751536

DO - 10.1109/NANO.2016.7751536

M3 - Conference contribution

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T3 - 16th International Conference on Nanotechnology - IEEE NANO 2016

SP - 681

EP - 682

BT - 16th International Conference on Nanotechnology - IEEE NANO 2016

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 16th IEEE International Conference on Nanotechnology - IEEE NANO 2016

Y2 - 22 August 2016 through 25 August 2016

ER -

Wang Z, Li J, Kimura M, Ono T. Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor. In 16th International Conference on Nanotechnology - IEEE NANO 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 681-682. 7751536. (16th International Conference on Nanotechnology - IEEE NANO 2016). doi: 10.1109/NANO.2016.7751536

Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor

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