Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry

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Abstract

About 150 modifications have been identified in RNA species. Besides their regulatory roles in the intracellular gene expression, abundant modified RNA nucleosides are catabolized from RNA and released into extracellular fluids, which can impact extracellular signaling as ligands for receptors. Here, we describe a protocol to prepare samples from biological specimens, including cultured cells, extracellular fluid, and tissues, to measure both intracellular and extracellular RNA modifications using mass spectrometry. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2021).

Original languageEnglish
Article number100848
JournalSTAR Protocols
Volume2
Issue number4
DOIs
Publication statusPublished - 2021 Dec 17

Keywords

  • Mass Spectrometry
  • Metabolism
  • Metabolomics
  • Molecular Biology
  • Signal Transduction

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