TY - JOUR
T1 - Psychophysiological stress induces heat shock cognate protein (HSC)70 mRNA in the cerebral cortex and stomach of rats
AU - Fukudo, Shin
AU - Abe, Koji
AU - Hongo, Michio
AU - Utsumi, Atsushi
AU - Itoyama, Yasuto
N1 - Funding Information:
This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan. We thank the staff at Word-Medex for their assistance in editing the manuscript.
PY - 1995/3/27
Y1 - 1995/3/27
N2 - Families of 70 kDa heat shock proteins have essential roles in cellular coping to noxious stimuli. However, their roles in psychophysiological stress have not been precisely clarified. We tested our hypothesis that heat shock cognate protein (HSC)70 messenger RNA would increase in stress-vulnerable organs under psychophysiological stress. In control rats, cerebral HSC70 mRNAs were constitutively expressed while gastric HSC70 mRNAs were scarcely identified. Restraint-water immersion stress significantly increased the level of cerebral HSC70 mRNAs for 6 h and 12 h. Stress for 6 h with recovery for 6 h induced more gastric HSC70 mRNA levels than that without recovery, while stress for 12 h expressed the highest gastric HCS70 mRNA levels. Hypothermia, induced by water immersion, excluded a possible role of hyperthermia in inducing HSC70 mRNA. Our results point to a crucial cytoprotective role for families of heat shock proteins in stress-vulnerable brain-gut link in mammals under psychophysiological stress.
AB - Families of 70 kDa heat shock proteins have essential roles in cellular coping to noxious stimuli. However, their roles in psychophysiological stress have not been precisely clarified. We tested our hypothesis that heat shock cognate protein (HSC)70 messenger RNA would increase in stress-vulnerable organs under psychophysiological stress. In control rats, cerebral HSC70 mRNAs were constitutively expressed while gastric HSC70 mRNAs were scarcely identified. Restraint-water immersion stress significantly increased the level of cerebral HSC70 mRNAs for 6 h and 12 h. Stress for 6 h with recovery for 6 h induced more gastric HSC70 mRNA levels than that without recovery, while stress for 12 h expressed the highest gastric HCS70 mRNA levels. Hypothermia, induced by water immersion, excluded a possible role of hyperthermia in inducing HSC70 mRNA. Our results point to a crucial cytoprotective role for families of heat shock proteins in stress-vulnerable brain-gut link in mammals under psychophysiological stress.
KW - Cerebral cortex
KW - Heat shock cognate protein 70
KW - Heat shock protein
KW - Messenger RNA
KW - Northern blotting
KW - Rat
KW - Restraint-water immersion stress
KW - Stomach
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U2 - 10.1016/0006-8993(95)00044-Q
DO - 10.1016/0006-8993(95)00044-Q
M3 - Article
C2 - 7796158
AN - SCOPUS:0028927971
SN - 0006-8993
VL - 675
SP - 98
EP - 102
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -