Purification and characterization of an agglutinin of the soft coral Sinularia species

Rina Goto; Koji Muramoto; Masatoshi Yamazaki; Hisao Kamiya

doi:10.1016/0145-305X(92)90047-G

Purification and characterization of an agglutinin of the soft coral Sinularia species

Rina Goto, Koji Muramoto, Masatoshi Yamazaki, Hisao Kamiya

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca⁺⁺-independent. Sinularian promoted binding of macrophages to tumor cells.

Original language	English
Pages (from-to)	9-17
Number of pages	9
Journal	Developmental and Comparative Immunology
Volume	16
Issue number	1
DOIs	https://doi.org/10.1016/0145-305X(92)90047-G
Publication status	Published - 1992
Externally published	Yes

Keywords

Agglutinin
Sinularia sp
Soft coral

ASJC Scopus subject areas

Immunology
Developmental Biology

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10.1016/0145-305X(92)90047-G

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title = "Purification and characterization of an agglutinin of the soft coral Sinularia species",

abstract = "A d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca++-independent. Sinularian promoted binding of macrophages to tumor cells.",

keywords = "Agglutinin, Sinularia sp, Soft coral",

author = "Rina Goto and Koji Muramoto and Masatoshi Yamazaki and Hisao Kamiya",

note = "Funding Information: Acknowledgments--This study was partly supported by a Grant-in-Aid for Scientific Research and Cancer Research from the Ministry of Education, Science and Culture of Japan and also by a grant from Nissan Science Foundation.",

year = "1992",

doi = "10.1016/0145-305X(92)90047-G",

language = "English",

volume = "16",

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journal = "Developmental and Comparative Immunology",

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TY - JOUR

T1 - Purification and characterization of an agglutinin of the soft coral Sinularia species

AU - Goto, Rina

AU - Muramoto, Koji

AU - Yamazaki, Masatoshi

AU - Kamiya, Hisao

N1 - Funding Information: Acknowledgments--This study was partly supported by a Grant-in-Aid for Scientific Research and Cancer Research from the Ministry of Education, Science and Culture of Japan and also by a grant from Nissan Science Foundation.

PY - 1992

Y1 - 1992

N2 - A d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca++-independent. Sinularian promoted binding of macrophages to tumor cells.

AB - A d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca++-independent. Sinularian promoted binding of macrophages to tumor cells.

KW - Agglutinin

KW - Sinularia sp

KW - Soft coral

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SN - 0145-305X

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JO - Developmental and Comparative Immunology

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Purification and characterization of an agglutinin of the soft coral Sinularia species

Abstract

Keywords

ASJC Scopus subject areas

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