A d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca++-independent. Sinularian promoted binding of macrophages to tumor cells.
|Number of pages||9|
|Journal||Developmental and Comparative Immunology|
|Publication status||Published - 1992|
- Sinularia sp
- Soft coral
ASJC Scopus subject areas
- Developmental Biology