Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis

Yuji Nagata; Takashi Hatta; Ryozo Imai; Kazuhide Kimbara; Masao Fukuda; Keiji Yano; Masamichi Takagi

doi:10.1271/bbb.57.1582

Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis

Yuji Nagata, Takashi Hatta, Ryozo Imai, Kazuhide Kimbara, Masao Fukuda, Keiji Yano, Masamichi Takagi

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The linA gene from Pseudomonas paucimobilis was highly expressed in Escherichia coli, and the linA product (LinA), named γ-HCH dehydrochlorinase, was purified to homogeneity. LinA released three chloride ions per one molecule of γ-HCH. Degradation assay of halogenated compounds by purified LinA showed that the substrate specificity of LinA is very narrow.

Original language	English
Pages (from-to)	1582-1583
Number of pages	2
Journal	Bioscience, Biotechnology and Biochemistry
Volume	57
Issue number	9
DOIs	https://doi.org/10.1271/bbb.57.1582
Publication status	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "The linA gene from Pseudomonas paucimobilis was highly expressed in Escherichia coli, and the linA product (LinA), named γ-HCH dehydrochlorinase, was purified to homogeneity. LinA released three chloride ions per one molecule of γ-HCH. Degradation assay of halogenated compounds by purified LinA showed that the substrate specificity of LinA is very narrow.",

author = "Yuji Nagata and Takashi Hatta and Ryozo Imai and Kazuhide Kimbara and Masao Fukuda and Keiji Yano and Masamichi Takagi",

year = "1993",

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language = "English",

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journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis

AU - Nagata, Yuji

AU - Hatta, Takashi

AU - Imai, Ryozo

AU - Kimbara, Kazuhide

AU - Fukuda, Masao

AU - Yano, Keiji

AU - Takagi, Masamichi

PY - 1993

Y1 - 1993

N2 - The linA gene from Pseudomonas paucimobilis was highly expressed in Escherichia coli, and the linA product (LinA), named γ-HCH dehydrochlorinase, was purified to homogeneity. LinA released three chloride ions per one molecule of γ-HCH. Degradation assay of halogenated compounds by purified LinA showed that the substrate specificity of LinA is very narrow.

AB - The linA gene from Pseudomonas paucimobilis was highly expressed in Escherichia coli, and the linA product (LinA), named γ-HCH dehydrochlorinase, was purified to homogeneity. LinA released three chloride ions per one molecule of γ-HCH. Degradation assay of halogenated compounds by purified LinA showed that the substrate specificity of LinA is very narrow.

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IS - 9

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Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis

Abstract

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