@article{3180a6540e5645779b865aad16ecdadc,
title = "Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy",
abstract = "Previous studies showed that gold nanoparticles (AuNPs) are useful radiosensitizers which optimize radiation therapy under low-dose radiation. However, the mechanisms of AuNP radiosensitization, including the amount and localization of the AuNPs interacting with cancer cells, has not yet been quantified. To answer these questions, we prepared AuNPs conjugated with anti-human epidermal growth factor receptor type 2 (HER2) antibody via polyethylene glycol (PEG) chains (AuNP-PEG-HER2ab). AuNP-PEG-HER2ab specifically bound to the HER2-expressing cancer cells and entered the cells via endocytosis. Whether endocytosis of AuNP-PEG-HER2ab occurred had no effect on radiosensitization efficacy by AuNP-PEG-HER2ab in vitro. The radiosensitization efficacy in vitro depended on dose of AuNP-PEG-HER2ab or dose of X-ray. Moreover, AuNP-PEG-HER2ab administrated into tumor-bearing mice was localized to both the periphery of the tumor tissue and near the nuclei in cancer cells in tumor deep tissue. The localization of AuNP-PEG-HER2ab in tumor tissues was important factors for in vivo powerful radiosensitization efficacy.",
keywords = "Gold nanoparticle, HER2, Low-dose radiation, Radiation therapy, Radiosensitizer",
author = "Keiichiro Hatoyama and Narufumi Kitamura and Mayumi Takano-Kasuya and Masayuki Tokunaga and Takahiro Oikawa and Mineto Ohta and Yoh Hamada and Hiroshi Tada and Yoshio Kobayashi and Takashi Kamei and Kohsuke Gonda",
note = "Funding Information: We thank Y. Furukawa for technical assistance in the preparation of pathological sections and M. Kaji for technical assistance in Transmission electron microscopy imaging. We also acknowledge the support of the Biomedical Research Core of Tohoku University Graduate School of Medicine and Konica Minolta, Inc . A portion of this work was supported by a Grant-in-Aid for Scientific Research (B) ( 16H05168 ) from the Japan Society for the Promotion of Science , a Grant-in-Aid for the A3 Foresight Program from the Japan Society for the Promotion of Science , and a Grant-in-Aid for Young Scientists (B) ( 17K15767 ) from the Japan Society for the Promotion of Science . Funding Information: We thank Y. Furukawa for technical assistance in the preparation of pathological sections and M. Kaji for technical assistance in Transmission electron microscopy imaging. We also acknowledge the support of the Biomedical Research Core of Tohoku University Graduate School of Medicine and Konica Minolta, Inc. A portion of this work was supported by a Grant-in-Aid for Scientific Research (B) (16H05168) from the Japan Society for the Promotion of Science, a Grant-in-Aid for the A3 Foresight Program from the Japan Society for the Promotion of Science, and a Grant-in-Aid for Young Scientists (B) (17K15767) from the Japan Society for the Promotion of Science. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2019",
month = jan,
day = "22",
doi = "10.1016/j.bbrc.2018.12.016",
language = "English",
volume = "508",
pages = "1093--1100",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",
}
