TY - JOUR
T1 - Quantitative liquid chromatography-tandem mass spectrometric analysis of 11-dehydroTXB2 in urine
AU - Suzuki, Naoto
AU - Hishinuma, Takanori
AU - Chiba, Satoko
AU - Saga, Toshihide
AU - Tsukamoto, Hiroki
AU - Mizugaki, Michinao
AU - Goto, Junichi
PY - 2004/1/1
Y1 - 2004/1/1
N2 - A simple and selective determination method of 11-dehydrothromboxane B 2 (11-dehydroTXB2), which is urinary metabolite of TXA2, has been developed employing liquid chromatography-tandem mass spectrometry (LC-MS-MS). 11-DehydroTXB2 and its deuterium-labeled analogue as an internal standard were extracted from urine by simple solid-phase extraction (SPE). These compounds were analyzed using LC-MS-MS in the selected reaction monitoring (SRM) mode, by monitoring the transitions from m/z 367 to m/z 161 for 11-dehydroTXB2 and from m/z 371 to m/z 165 for its internal standard. A good linear response over the range 50pg-10ng per tube was demonstrated. The values determined by LC-MS-MS were well validated and closely corresponded to the values determined by gas chromatography-mass spectrometry (GC-MS). The mean concentration of 11-dehydroTXB2 in urine of healthy adults was 635±427pg/mg creatinine (mean±S.D.,n= 13). This simple, accurate and selective determination method described in this study should greatly aid in evaluating the role of TXA2 in vivo.
AB - A simple and selective determination method of 11-dehydrothromboxane B 2 (11-dehydroTXB2), which is urinary metabolite of TXA2, has been developed employing liquid chromatography-tandem mass spectrometry (LC-MS-MS). 11-DehydroTXB2 and its deuterium-labeled analogue as an internal standard were extracted from urine by simple solid-phase extraction (SPE). These compounds were analyzed using LC-MS-MS in the selected reaction monitoring (SRM) mode, by monitoring the transitions from m/z 367 to m/z 161 for 11-dehydroTXB2 and from m/z 371 to m/z 165 for its internal standard. A good linear response over the range 50pg-10ng per tube was demonstrated. The values determined by LC-MS-MS were well validated and closely corresponded to the values determined by gas chromatography-mass spectrometry (GC-MS). The mean concentration of 11-dehydroTXB2 in urine of healthy adults was 635±427pg/mg creatinine (mean±S.D.,n= 13). This simple, accurate and selective determination method described in this study should greatly aid in evaluating the role of TXA2 in vivo.
KW - 11-Dehydrothromboxane B
KW - Liquid chromatography-tandem mass spectrometry
KW - Thromboxane A
UR - http://www.scopus.com/inward/record.url?scp=1842453340&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842453340&partnerID=8YFLogxK
U2 - 10.1016/j.prostaglandins.2004.01.003
DO - 10.1016/j.prostaglandins.2004.01.003
M3 - Article
C2 - 15165035
AN - SCOPUS:1842453340
SN - 0090-6980
VL - 73
SP - 103
EP - 110
JO - Journal of Lipid Mediators
JF - Journal of Lipid Mediators
IS - 1-2
ER -