Raf-1 is a binding partner of DSCR1

Young Jin Cho, Mayumi Abe, Seong Yun Kim, Yasufumi Sato

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Down syndrome critical region 1 (DSCR1) is recognized as an endogenous calcineurin inhibitor. DSCR1 is induced in endothelial cells and may play an important role in inflammation and angiogenesis. To address a novel function of DSCR1, we searched interacting partners of DSCR1. We performed pull-down analysis using DSCR1 as a bait and identified Raf-1 as a binding partner. The association of Raf-1 was confirmed by co-immunoprecipitation in GM7373 cells expressing green fluorescence protein tagged DSCR1. We determined two Raf-1 binding regions in DSCR1; one in the N-terminus and the other in the C-terminus regions. We further demonstrated that calpain cleaved DSCR1 and generated fragments with different binding affinity to Raf-1 or calcineurin. These results constitute the first demonstration of Raf-1 as a binding partner of DSCR1, and suggest a novel role of DSCR1.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalArchives of Biochemistry and Biophysics
Issue number1
Publication statusPublished - 2005 Jul 1


  • Angiogenesis
  • DSCR1
  • Endothelial cell
  • Raf-1


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