Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique

Samad Ahadian; Shukuyo Yamada; Javier Ramón-Azcón; Kosuke Ino; Hitoshi Shiku; Ali Khademhosseini; Tomokazu Matsue

doi:10.1039/c4lc00479e

Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique

Samad Ahadian, Shukuyo Yamada, Javier Ramón-Azcón, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

In this manuscript, we demonstrate the rapid formation of three-dimensional (3D) embryonic stem cell (ESC) aggregates with controllable sizes and shapes in hydrogels using dielectrophoresis (DEP). The ESCs encapsulated within a methacrylated gelatin (GelMA) prepolymer were introduced into a DEP device and, upon applying an electric field and crosslinking of the GelMA hydrogel, formed 3D ESC aggregates. Embryoid bodies (EBs) fabricated using this method showed high cellular viability and pluripotency. The proposed technique enables production of EBs on a large scale and in a high-throughput manner for potential cell therapy and tissue regeneration applications.

Original language	English
Pages (from-to)	3690-3694
Number of pages	5
Journal	Lab on a Chip
Volume	14
Issue number	19
DOIs	https://doi.org/10.1039/c4lc00479e
Publication status	Published - 2014

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abstract = "In this manuscript, we demonstrate the rapid formation of three-dimensional (3D) embryonic stem cell (ESC) aggregates with controllable sizes and shapes in hydrogels using dielectrophoresis (DEP). The ESCs encapsulated within a methacrylated gelatin (GelMA) prepolymer were introduced into a DEP device and, upon applying an electric field and crosslinking of the GelMA hydrogel, formed 3D ESC aggregates. Embryoid bodies (EBs) fabricated using this method showed high cellular viability and pluripotency. The proposed technique enables production of EBs on a large scale and in a high-throughput manner for potential cell therapy and tissue regeneration applications.",

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AU - Ahadian, Samad

AU - Yamada, Shukuyo

AU - Ramón-Azcón, Javier

AU - Ino, Kosuke

AU - Shiku, Hitoshi

AU - Khademhosseini, Ali

AU - Matsue, Tomokazu

PY - 2014

Y1 - 2014

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AB - In this manuscript, we demonstrate the rapid formation of three-dimensional (3D) embryonic stem cell (ESC) aggregates with controllable sizes and shapes in hydrogels using dielectrophoresis (DEP). The ESCs encapsulated within a methacrylated gelatin (GelMA) prepolymer were introduced into a DEP device and, upon applying an electric field and crosslinking of the GelMA hydrogel, formed 3D ESC aggregates. Embryoid bodies (EBs) fabricated using this method showed high cellular viability and pluripotency. The proposed technique enables production of EBs on a large scale and in a high-throughput manner for potential cell therapy and tissue regeneration applications.

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Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique

Abstract

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