@article{80b36cde7cfd48128571fea03c77439e,
title = "Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer",
abstract = "A rapid, facile and selective detection of anti-H5 subtype avian influenza virus (AIV) antibody in serum by fluorescence polarization immunoassay (FPIA) was achieved. A fragment of recombinant H5 subtype AIV hemagglutinin was produced and labeled with fluorescein to use it as a labeled antigen in FPIA. This labeled antigen was mixed with anti-AIV sera (H1–H16 subtypes) and FP of the mixture was measured using a portable FP analyzer on a microdevice. It was found that FP increased in proportion to the concentration of anti-H5 AIV antibody (serum) and was significantly higher than FP obtained with the other sera. The selective detection of anti-H5 subtype AIV antibody was confirmed. The required volume of original sample was 2 μL and analysis time was within 20 min. This detection system realizes an efficient on-site diagnosis and surveillance of AIV.",
keywords = "Antibody detection, Avian influenza, Fluorescence polarization immunoassay, Rapid diagnosis",
author = "Keine Nishiyama and Yohei Takeda and Masatoshi Maeki and Akihiko Ishida and Hirofumi Tani and Koji Shigemura and Akihide Hibara and Yutaka Yonezawa and Kunitoshi Imai and Haruko Ogawa and Manabu Tokeshi",
note = "Funding Information: This work was supported by the JST-SENTAN program JPMJSN16A2 . This work was partially supported by Hokkaido University Research and Education Center for Robust Agriculture, Forestry, and Fisheries Industry . This research and development work was also supported by the MEXT Doctoral program for Data-Related InnoVation Expert Hokkaido University (D-DRIVE-HU) program . We would like to thank Dr. Toshihiro Ito, Tottori University, Japan, who kindly provided us with the H5N3 AIV; and Dr. Yoshihiro Sakoda, Hokkaido University, Japan, who kindly provided us with the anti-H1–H16 sera. Funding Information: This work was supported by the JST-SENTAN programJPMJSN16A2. This work was partially supported by Hokkaido University Research and Education Center for Robust Agriculture, Forestry, and Fisheries Industry. This research and development work was also supported by the MEXT Doctoral program for Data-Related InnoVation Expert Hokkaido University (D-DRIVE-HU) program. We would like to thank Dr. Toshihiro Ito, Tottori University, Japan, who kindly provided us with the H5N3 AIV; and Dr. Yoshihiro Sakoda, Hokkaido University, Japan, who kindly provided us with the anti-H1?H16 sera. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",
year = "2020",
month = aug,
day = "1",
doi = "10.1016/j.snb.2020.128160",
language = "English",
volume = "316",
journal = "Sensors and Actuators B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
}