Rapid separation and manipulation of DNA by a Ratcheting Electrophoresis Microchip (REM)

Katherine Dunphy, Veljko Milanovic, Samantha Andrews, Taku Ohara, Arun Majumdar

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

The Ratcheting Electrophoresis Microchip (REM) is a microfluidic device for electrophoretic separation of biomolecules such as DNA and proteins. By using thousands of electrodes along the length of a microchannel, the REM separates molecules using low applied voltages (∼1 V) in short times (< 1 minute). This paper describes the microfabriation of the REM and initial testing results. Parallel arrays of platinum electrodes are fabricated on a silicon chip with a pitch of 10 μm. Two types of channels are fabricated: silicon nitride channels fabricated on the chip and poly(dimelthylsiloxane) (PDMS) channels fabricated separately and attached to the chip. Initial testing shows partial success with the PDMS channels and promising results for the silicon nitride channels.

Original languageEnglish
Title of host publicationFluids Engineering
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages419-423
Number of pages5
ISBN (Print)0791836576, 9780791836576
DOIs
Publication statusPublished - 2002

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings

Keywords

  • BioMEMS
  • DNA
  • Electrophoresis
  • Microchip
  • Ratchet

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