TY - JOUR
T1 - Rat-derived feeder cells immortalized by expression of mutant CDK4, cyclin D, and telomerase can support stem cell growth
AU - Katayama, Masafumi
AU - Kiyono, Tohru
AU - Kuroda, Kengo
AU - Ueda, Kazuma
AU - Onuma, Manabu
AU - Shirakawa, Hitoshi
AU - Fukuda, Tomokazu
N1 - Funding Information:
We would like to thank Prof. Emiko Isogai (Graduate School of Agricultural Science, Tohoku University) for assistance with cell cycle analysis. We would also like to thank Dr. Hiroyuki Miyoshi (RIKEN Bio Resource Center, presently at Keio University) for providing us with lentivirus plasmid vectors. Masafumi Katayama: MK was involved in the study design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing. Tohru Kiyono: TK provided experimental material and was involved in the final approval of the manuscript. Kengo Kuroda: KK provided experimental material. Kazuma Ueda: KU provided experimental material. Manabu Onuma: MO provided experimental material. Hitoshi Shirakawa: HS provided experimental material. Tomokazu Fukuda: TF was involved in the study design, financial support, collection and/or assembly of data, manuscript writing, and final approval of the manuscript. The authors declare there are no conflicts of interest.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/5
Y1 - 2019/5
N2 - The maintenance of stem cells often requires the support of feeder cells. Primary mouse embryonic fibroblasts (MEFs) have traditionally been used as feeder cells, and although these MEF-derived feeder cells have exhibited a reasonable performance, they require repeated cell isolation, since MEFs cannot expand indefinitely. To overcome this limitation, immortalized cells, such as STO cells, have been used. However, one major disadvantage is that previously reported immortalized cells can only support stem cell cultures for a relatively short period, typically 4 to 7 days. In this study, we found that our newly established rat-derived fibroblasts immortalized by the expression of mutant cyclin-dependent kinase 4, cyclin D, and telomerase reverse transcriptase, can function as feeder cells for relatively long cell culture periods of approximately 14 days. The rat-derived immortalized cells developed in this study should be a useful source of feeder cells to support stem cell research.
AB - The maintenance of stem cells often requires the support of feeder cells. Primary mouse embryonic fibroblasts (MEFs) have traditionally been used as feeder cells, and although these MEF-derived feeder cells have exhibited a reasonable performance, they require repeated cell isolation, since MEFs cannot expand indefinitely. To overcome this limitation, immortalized cells, such as STO cells, have been used. However, one major disadvantage is that previously reported immortalized cells can only support stem cell cultures for a relatively short period, typically 4 to 7 days. In this study, we found that our newly established rat-derived fibroblasts immortalized by the expression of mutant cyclin-dependent kinase 4, cyclin D, and telomerase reverse transcriptase, can function as feeder cells for relatively long cell culture periods of approximately 14 days. The rat-derived immortalized cells developed in this study should be a useful source of feeder cells to support stem cell research.
KW - Cell cycle arrest
KW - Feeder cell
KW - Immortalized cell
KW - Mitomycin C
KW - Species difference
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U2 - 10.1016/j.bbamcr.2019.02.013
DO - 10.1016/j.bbamcr.2019.02.013
M3 - Article
C2 - 30826331
AN - SCOPUS:85062690189
SN - 0167-4889
VL - 1866
SP - 945
EP - 956
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 5
ER -