Ratiometric fluorescent signaling of small molecule, environmentally sensitive dye conjugates for detecting single-base mutations in DNA

Chun Xia Wang; Yusuke Sato; Megumi Kudo; Seiichi Nishizawa; Norio Teramae

doi:10.1002/chem.201200219

Ratiometric fluorescent signaling of small molecule, environmentally sensitive dye conjugates for detecting single-base mutations in DNA

Chun Xia Wang, Yusuke Sato, Megumi Kudo, Seiichi Nishizawa, Norio Teramae

SCI - Chemistry

Tohoku University

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Bind it! A ratiometric fluorescent probe based on conjugation of a DNA-binding ligand with an environmentally sensitive dye to probe hydrophobic grooves of the DNA helix was developed. The resulting conjugate ATMND-DBD can bind to an orphan nucleobase opposite an abasic (AP) site with high selectivity and affinity for pyrimidine bases (see figure).

Original language	English
Pages (from-to)	9481-9484
Number of pages	4
Journal	Chemistry - A European Journal
Volume	18
Issue number	31
DOIs	https://doi.org/10.1002/chem.201200219
Publication status	Published - 2012 Jul 27

Keywords

DNA recognition
fluorescent probes
ligand design
noncovalent interactions
nucleobases

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10.1002/chem.201200219

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abstract = "Bind it! A ratiometric fluorescent probe based on conjugation of a DNA-binding ligand with an environmentally sensitive dye to probe hydrophobic grooves of the DNA helix was developed. The resulting conjugate ATMND-DBD can bind to an orphan nucleobase opposite an abasic (AP) site with high selectivity and affinity for pyrimidine bases (see figure).",

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AU - Wang, Chun Xia

AU - Sato, Yusuke

AU - Kudo, Megumi

AU - Nishizawa, Seiichi

AU - Teramae, Norio

PY - 2012/7/27

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KW - DNA recognition

KW - fluorescent probes

KW - ligand design

KW - noncovalent interactions

KW - nucleobases

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Ratiometric fluorescent signaling of small molecule, environmentally sensitive dye conjugates for detecting single-base mutations in DNA

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Keywords

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