@article{9d26c26bc5fa40719063c249e76959e8,
title = "Ratiometric imaging of intracellular Mg2+ dynamics using a red fluorescent turn-off probe and a green fluorescent turn-on probe",
abstract = "In this work, we developed a red fluorescent turn-off Mg2+ probe with high Mg2+ selectivity. The quenching response to Mg2+ in combination with a green fluorescent turn-on Mg2+ probe enabled a ratiometric imaging of the changes in intracellular Mg2+ concentration. This ratiometric imaging system significantly enhanced the detection sensitivity of intracellular Mg2+ fluctuations in comparison with a conventional ratiometric Mg2+ sensing system, thus leading to a valuable technique for visualizing small change in intracellular Mg2+ concentration.",
keywords = "Intracellular Mg concentration, Ratiometric imaging, Red fluorescent turn-off probe",
author = "Yusuke Matsui and Shin Mizukami and Kazuya Kikuchi",
note = "Funding Information: This research was supported by the Grant-in-Aid for Scientific Research (Grant Nos. 24651259, 25220207, 15H03120, 15H00818, and 16K13102), and Innovative Areas “Molecular Robotics” (No. 24104004) and “Dynamic Ordering & Integrated Functions” (No. 16H00768) of The MEXT, Japan; the JSPS through its Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program); the MHLW, Japan and CREST from JST; Suzuken Memorial Foundation; the Uehara Memorial Foundation; and Takeda Science Foundation. We also thank Prof. Hiroaki Miki and Dr. Yosuke Funato (Research Institute for Microbial Diseases, Osaka Univ.) for helpful discussions and for providing the CNNM4 gene. Publisher Copyright: {\textcopyright} 2018 The Chemical Society of Japan.",
year = "2018",
doi = "10.1246/cl.170918",
language = "English",
volume = "47",
pages = "23--26",
journal = "Chemistry Letters",
issn = "0366-7022",
publisher = "Chemical Society of Japan",
number = "1",
}