Rearrangement of the retinoic acid receptor gene in acute promyelocytic leukemia

Kun Sang Chang; Jose M. Trujillo; Toshihiko Ogura; C. M. Castiglione; Kenneth K. Kidd; Shourong Zhao; Emil J. Freireich; Sanford A. Stass

Rearrangement of the retinoic acid receptor gene in acute promyelocytic leukemia

Kun Sang Chang, Jose M. Trujillo, Toshihiko Ogura, C. M. Castiglione, Kenneth K. Kidd, Shourong Zhao, Emil J. Freireich, Sanford A. Stass

IDAC - Division of Brain Science

Research output: Contribution to journal › Article › peer-review

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Abstract

The retinoic acid receptor-alpha (RAR-α) gene was previously localized to chromosome 17q21, a region close to the t(15;17) (q22;q21) abnormality in acute promyelocytic leukemia (APL). We used the RAR-α gene as a probe and found that eight of nine APL patient samples with t(15;17) (q22;q21) showed rearranged bands. A tenth APL patient was diploid and demonstrated no rearrangement. One patient who had rearrangement as an acute leukemia did not have rearrangement in remission. The results obtained from intron/exon mapping of the RAR-α gene demonstrated that breakpoints of seven of the eight patients occurred within intron 1. Northern blot analysis of leukemic samples indicated the expression of two RAR-α mRNA of 2.7 and 3.7 kb. However, two additional mRNA of 4.1 and 3.2 kb were found in an APL patient. We conclude that the RAR-α gene is directly involved in the t(15;17) translocation in APL and may transcribe aberrant messages.

Original language	English
Pages (from-to)	200-204
Number of pages	5
Journal	Leukemia
Volume	5
Issue number	3
Publication status	Published - 1991 Mar

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title = "Rearrangement of the retinoic acid receptor gene in acute promyelocytic leukemia",

abstract = "The retinoic acid receptor-alpha (RAR-α) gene was previously localized to chromosome 17q21, a region close to the t(15;17) (q22;q21) abnormality in acute promyelocytic leukemia (APL). We used the RAR-α gene as a probe and found that eight of nine APL patient samples with t(15;17) (q22;q21) showed rearranged bands. A tenth APL patient was diploid and demonstrated no rearrangement. One patient who had rearrangement as an acute leukemia did not have rearrangement in remission. The results obtained from intron/exon mapping of the RAR-α gene demonstrated that breakpoints of seven of the eight patients occurred within intron 1. Northern blot analysis of leukemic samples indicated the expression of two RAR-α mRNA of 2.7 and 3.7 kb. However, two additional mRNA of 4.1 and 3.2 kb were found in an APL patient. We conclude that the RAR-α gene is directly involved in the t(15;17) translocation in APL and may transcribe aberrant messages.",

author = "Chang, {Kun Sang} and Trujillo, {Jose M.} and Toshihiko Ogura and Castiglione, {C. M.} and Kidd, {Kenneth K.} and Shourong Zhao and Freireich, {Emil J.} and Stass, {Sanford A.}",

year = "1991",

month = mar,

language = "English",

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T1 - Rearrangement of the retinoic acid receptor gene in acute promyelocytic leukemia

AU - Chang, Kun Sang

AU - Trujillo, Jose M.

AU - Ogura, Toshihiko

AU - Castiglione, C. M.

AU - Kidd, Kenneth K.

AU - Zhao, Shourong

AU - Freireich, Emil J.

AU - Stass, Sanford A.

PY - 1991/3

Y1 - 1991/3

N2 - The retinoic acid receptor-alpha (RAR-α) gene was previously localized to chromosome 17q21, a region close to the t(15;17) (q22;q21) abnormality in acute promyelocytic leukemia (APL). We used the RAR-α gene as a probe and found that eight of nine APL patient samples with t(15;17) (q22;q21) showed rearranged bands. A tenth APL patient was diploid and demonstrated no rearrangement. One patient who had rearrangement as an acute leukemia did not have rearrangement in remission. The results obtained from intron/exon mapping of the RAR-α gene demonstrated that breakpoints of seven of the eight patients occurred within intron 1. Northern blot analysis of leukemic samples indicated the expression of two RAR-α mRNA of 2.7 and 3.7 kb. However, two additional mRNA of 4.1 and 3.2 kb were found in an APL patient. We conclude that the RAR-α gene is directly involved in the t(15;17) translocation in APL and may transcribe aberrant messages.

AB - The retinoic acid receptor-alpha (RAR-α) gene was previously localized to chromosome 17q21, a region close to the t(15;17) (q22;q21) abnormality in acute promyelocytic leukemia (APL). We used the RAR-α gene as a probe and found that eight of nine APL patient samples with t(15;17) (q22;q21) showed rearranged bands. A tenth APL patient was diploid and demonstrated no rearrangement. One patient who had rearrangement as an acute leukemia did not have rearrangement in remission. The results obtained from intron/exon mapping of the RAR-α gene demonstrated that breakpoints of seven of the eight patients occurred within intron 1. Northern blot analysis of leukemic samples indicated the expression of two RAR-α mRNA of 2.7 and 3.7 kb. However, two additional mRNA of 4.1 and 3.2 kb were found in an APL patient. We conclude that the RAR-α gene is directly involved in the t(15;17) translocation in APL and may transcribe aberrant messages.

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AN - SCOPUS:0025825266

SN - 0887-6924

VL - 5

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JO - Leukemia

JF - Leukemia

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ER -

Rearrangement of the retinoic acid receptor gene in acute promyelocytic leukemia

Abstract

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