Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest

Kazushige Kuroha; Mayuko Akamatsu; Lyudmila Dimitrova; Takehiko Ito; Yuki Kato; Katsuhiko Shirahige; Toshifumi Inada

doi:10.1038/embor.2010.169

Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest

Kazushige Kuroha, Mayuko Akamatsu, Lyudmila Dimitrova, Takehiko Ito, Yuki Kato, Katsuhiko Shirahige, Toshifumi Inada

Research output: Contribution to journal › Article › peer-review

120 Citations (Scopus)

Abstract

Nascent peptide-dependent translation arrest is crucial for the quality control of eukaryotic gene expression. Here we show that the receptor for activated C kinase 1 (RACK1) participates in nascent peptide-dependent translation arrest, and that its binding to the 40S subunit is crucial for this. Translation arrest by a nascent peptide results in Dom34/Hbs1-independent endonucleolytic cleavage of mRNA, and this is stimulated by RACK1. We propose that RACK1 stimulates the translation arrest that is induced by basic amino-acid sequences that leads to endonucleolytic cleavage of the mRNA, as well as to co-translational protein degradation.

Original language	English
Pages (from-to)	956-961
Number of pages	6
Journal	EMBO Reports
Volume	11
Issue number	12
DOIs	https://doi.org/10.1038/embor.2010.169
Publication status	Published - 2010 Dec
Externally published	Yes

Keywords

RACK1
endonucleolytic cleavage
mRNA quality control
nascent peptide
translation arrest

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.1038/embor.2010.169

Cite this

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abstract = "Nascent peptide-dependent translation arrest is crucial for the quality control of eukaryotic gene expression. Here we show that the receptor for activated C kinase 1 (RACK1) participates in nascent peptide-dependent translation arrest, and that its binding to the 40S subunit is crucial for this. Translation arrest by a nascent peptide results in Dom34/Hbs1-independent endonucleolytic cleavage of mRNA, and this is stimulated by RACK1. We propose that RACK1 stimulates the translation arrest that is induced by basic amino-acid sequences that leads to endonucleolytic cleavage of the mRNA, as well as to co-translational protein degradation.",

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T1 - Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest

AU - Kuroha, Kazushige

AU - Akamatsu, Mayuko

AU - Dimitrova, Lyudmila

AU - Ito, Takehiko

AU - Kato, Yuki

AU - Shirahige, Katsuhiko

AU - Inada, Toshifumi

PY - 2010/12

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AB - Nascent peptide-dependent translation arrest is crucial for the quality control of eukaryotic gene expression. Here we show that the receptor for activated C kinase 1 (RACK1) participates in nascent peptide-dependent translation arrest, and that its binding to the 40S subunit is crucial for this. Translation arrest by a nascent peptide results in Dom34/Hbs1-independent endonucleolytic cleavage of mRNA, and this is stimulated by RACK1. We propose that RACK1 stimulates the translation arrest that is induced by basic amino-acid sequences that leads to endonucleolytic cleavage of the mRNA, as well as to co-translational protein degradation.

KW - RACK1

KW - endonucleolytic cleavage

KW - mRNA quality control

KW - nascent peptide

KW - translation arrest

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Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest

Abstract

Keywords

ASJC Scopus subject areas

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