Recognition of artificial microstructures by sensory nerve fibers in culture

Takushi Hirono, Keiichi Torimitsu, Akio Kawana, Jun Fukuda

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65 Citations (Scopus)


Dissociated culture of adult mouse dorsal root ganglion cells on glass plates, on which grating-associated microstructures (a repetition of microgrooves [mGRV] and microsteps [mSTP] of 0.1-10 μm) are fabricated lithographic techniques, represents a remarkable bi-directional growth of their nerve fibers in the axial direction of the grating. Microscopical observations shows that the nerve fibers prefer to grow in the mGRV (70%), while their growth cones exhibit an even distribution onto the mGRV and mSTP. The efficiency of the nerve fibers to grow along the grating-axis are highly sensitive to a fine alteration of the width and depth of the mGRV. The preferential growth of the nerve fibers is thus due to a mechanical recognition of the microstructures by the growth cones and neurites.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalBrain Research
Issue number1
Publication statusPublished - 1988 Apr 12


  • Dorsal root ganglion neuron
  • Grating
  • Growth cone
  • Microstructure
  • Nerve cell culture
  • Neurite growth
  • Neurite guidance
  • Serum-free culture


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