Reduced HIC-1 gene expression in non-small cell lung cancer and its clinical significance

M. Hayashi, Y. Tokuchi, T. Hashimoto, S. I. Hayashi, K. Nishida, Y. Ishikawa, K. Nakagawa, S. Tsuchiya, S. Okumura, E. Tsuchiya

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Background: HIC-1 (hypermethylated in cancer-1) is a candidate tumor suppressor gene, identified in a region of frequent loss of heterozygosity on chromosome 17p13.3, which is telomeric from TP53 and often deleted in surgically resected lung cancers. To determine the significance of HIC-1 in lung cancer, we assessed its expression status and prognostic association in 47 adenocarcinomas and squamous cell carcinomas. Materials and Methods: RNA was extracted from tumors and corresponding normal tissues of surgically resected lungs, and the amount of HIC-1 mRNA was determined by means of semi-quantitative reverse transcriptase-polymerase chain reaction. Results: HIC-1 expression in tumors was less than that in normal lung tissues in 40 of 47 patients (85%), indicating frequent partial silencing. Median tumor/normal lung tissue (T/L) ratios for HIC-1 expression were 0.51 and 0.75 for adenocarcinomas and squamous cell carcinomas, respectively. No significant difference of median T/L ratio was observed between the two histological types, or among clinical stages of the patients. However, the reduced expression of HIC-1 gene in the tumor had a direct link with the clinical outcome: lower T/L ratios (<0.5) were significantly associated with short survival (P=0.034), an association also observed in cases restricted to stage I (P=0.047). Conclusions: The results suggest that low HIC-1 expression is involved in malignant progression of non-small cell lung cancer.

Original languageEnglish
Pages (from-to)535-540
Number of pages6
JournalAnticancer research
Issue number1 B
Publication statusPublished - 2001 Apr 17
Externally publishedYes


  • Chromosome 17p13.3
  • HIC-1 expression
  • Lung cancer
  • Prognosis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


Dive into the research topics of 'Reduced HIC-1 gene expression in non-small cell lung cancer and its clinical significance'. Together they form a unique fingerprint.

Cite this