Reduced host resistance and Th1 response to Cryptococcus neoformans in interleukin-18 deficient mice

Kazuyoshi Kawakami, Yoshinobu Koguchi, Mahboob Hossain Qureshi, Yuki Kinjo, Satomi Yara, Akiko Miyazato, Masashi Kurimoto, Kiyoshi Takeda, Shizuo Akira, Atsushi Saito

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


Using interleukin (IL)-18 deficient (IL-18(-/-)) mice, we examined the role of IL-18 in the host resistance and Th1 response against infection with Cryptococcus neoformans. Fungal clearance in the lung was reduced in IL-18(-/-) mice, although there was no significant change in the level of dissemination to the brain. The DTH response, as determined by footpad swelling, was also diminished in IL-18(-/-) mice compared to control wild-type (WT) mice. The levels of IL-12 and interferon (IFN)-γ in the sera were significantly lower in IL-18(-/-) mice than in WT mice. Spleen cells from infected WT mice produced a high level of IFN-γ upon stimulation with the microbe, while only a low level of IFN-γ production was detected in spleen cells from infected IL-18(-/-) mice. Administration of IL-18 almost completely restored the reduced response in IL-18(-/-) mice, while IL-12 showed a marginal effect. These results demonstrated the important role of IL-18 in the resistance and Th1 response of mice to C. neoformans by potentiating the production of IFN-γ. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - 2000 May 1
Externally publishedYes


  • Cryptococcus neoformans
  • DTH
  • IL-12
  • IL-18
  • Th1 response

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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