Reduction of laser-induced choroidal neovascularization by intravitreal vasohibin-1 in monkey eyes

Hideyuki Onami, Nobuhiro Nagai, Shigeki MacHida, Norihiro Kumasaka, Ryosuke Wakusawa, Yumi Ishikawa, Hikaru Sonoda, Yasufumi Sato, Toshiaki Abe

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9 Citations (Scopus)


PURPOSE: To determine whether intravitreal vasohibin-1 will reduce the grade of the choroidal neovascularization in monkey eyes. METHODS: Choroidal neovascularizations were induced in 12 monkey eyes by laser photocoagulation. Three monkeys were evaluated for the safety of the vasohibin-1 injections, 6 monkeys for the effects of a single injection, and 3 monkeys for repeated injections of vasohibin-1. Ophthalmoscopy, fluorescein angiography, focal electroretinograms, and optical coherence tomography were used for the evaluations. The level of vascular endothelial growth factor in the aqueous was determined by enzyme-linked immunosorbent assay. Immunohistochemistry was performed. RESULTS: An intravitreal injection of 10 μg of vasohibin-1 induced mild intraocular inflammation. Eyes with an intravitreal injection of 0.1 μg and 1.0 μg of vasohibin-1 had significant less fluorescein leakage from the choroidal neovascularizations and larger amplitude focal electroretinograms than that of vehicle-injected eyes. Similar results were obtained by repeated injections of 0.1 μg of vasohibin-1. Immunohistochemistry showed that vasohibin-1 was expressed mainly in the endothelial cells within the choroidal neovascularizations. The vascular endothelial growth factor level was not significantly altered by intravitreal vasohibin-1. CONCLUSION: The reduction of the laser-induced choroidal neovascularizations and preservation of macular function in monkey by intravitreal vasohibin-1 suggest that it should be considered for suppressing choroidal neovascularizations in humans.

Original languageEnglish
Pages (from-to)1204-1213
Number of pages10
Issue number6
Publication statusPublished - 2012 Jun


  • choroidal neovascularization
  • laserinduced
  • monkey
  • vascular endothelial growth factor
  • vasohibin-1


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