TY - JOUR
T1 - Regeneration of Sarcophaga imaginal discs in vitro
T2 - Implication of 20-hydroxyecdysone
AU - Kunieda, Takekazu
AU - Kurata, Shoichiro
AU - Natori, Shunji
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Speci®cally Promoted Research from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 1997/3/1
Y1 - 1997/3/1
N2 - When the 3/4 sectors of leg imaginal discs of Surcophaga were cultured in vitro in the presence of 2.5 x 10-8 M 20-hydroxyecdysone, wound healing and restoration of their morphology occurred. This concentration of ecdysone was critical for wound healing and was 40 times lower than that necessary for inducing differentiation of imaginal discs in vitro. Lost positional values revealed by expression of the wingless gene were found to show partial recovery under these conditions. These results suggest that a low titer of ecdysone is essential for the regeneration of imaginal discs.
AB - When the 3/4 sectors of leg imaginal discs of Surcophaga were cultured in vitro in the presence of 2.5 x 10-8 M 20-hydroxyecdysone, wound healing and restoration of their morphology occurred. This concentration of ecdysone was critical for wound healing and was 40 times lower than that necessary for inducing differentiation of imaginal discs in vitro. Lost positional values revealed by expression of the wingless gene were found to show partial recovery under these conditions. These results suggest that a low titer of ecdysone is essential for the regeneration of imaginal discs.
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U2 - 10.1006/dbio.1996.8498
DO - 10.1006/dbio.1996.8498
M3 - Article
C2 - 9119117
AN - SCOPUS:0031106035
SN - 0012-1606
VL - 183
SP - 86
EP - 94
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -