Regeneration of Sarcophaga imaginal discs in vitro: Implication of 20-hydroxyecdysone

Takekazu Kunieda, Shoichiro Kurata, Shunji Natori

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


When the 3/4 sectors of leg imaginal discs of Surcophaga were cultured in vitro in the presence of 2.5 x 10-8 M 20-hydroxyecdysone, wound healing and restoration of their morphology occurred. This concentration of ecdysone was critical for wound healing and was 40 times lower than that necessary for inducing differentiation of imaginal discs in vitro. Lost positional values revealed by expression of the wingless gene were found to show partial recovery under these conditions. These results suggest that a low titer of ecdysone is essential for the regeneration of imaginal discs.

Original languageEnglish
Pages (from-to)86-94
Number of pages9
JournalDevelopmental Biology
Issue number1
Publication statusPublished - 1997 Mar 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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