TY - JOUR
T1 - Regional difference in stainability with calcium-sensitive acetoxymethyl-ester probes in mouse brain slices
AU - Namiki, Shigehiro
AU - Sasaki, Takuya
AU - Matsuki, Norio
AU - Ikegaya, Yuji
N1 - Funding Information:
We are grateful to thank members of our laboratory for helpful discussion. This work was supported in part by a Grant-in-Aid for Science Research on Priority Areas (Elucidation of Neural Network Function in the Brain, numbers 18021008 and 17023015), Grants-in-Aid for Science Research 17650090 and 17689004 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Sumitomo Foundation Grant 050038.
PY - 2009/2
Y1 - 2009/2
N2 - Loading neurons with membrane permeable Ca2+ indicators is a core experimental procedure in functional multineuron Ca2+ imaging (fMCI), an optical technique for monitoring multiple neuronal activities. Although fMCI has been applied to several brain networks, including cerebral cortex, hippocampus, and cerebellum, no studies have systematically addressed the dye-loading efficiency in different brain regions. Here, we describe the stainability of Oregon Green 488 BAPTA-1AM in mouse acute brain slice preparations. The data are suggestive of the potential usability of fMCI in many brain regions, including olfactory bulb, thalamus, dentate gyrus, habenular nucleus, and pons.
AB - Loading neurons with membrane permeable Ca2+ indicators is a core experimental procedure in functional multineuron Ca2+ imaging (fMCI), an optical technique for monitoring multiple neuronal activities. Although fMCI has been applied to several brain networks, including cerebral cortex, hippocampus, and cerebellum, no studies have systematically addressed the dye-loading efficiency in different brain regions. Here, we describe the stainability of Oregon Green 488 BAPTA-1AM in mouse acute brain slice preparations. The data are suggestive of the potential usability of fMCI in many brain regions, including olfactory bulb, thalamus, dentate gyrus, habenular nucleus, and pons.
KW - Acetoxymethyl ester
KW - Calcium imaging
KW - fMCI
KW - Mouse
KW - Neuron
KW - Slice
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U2 - 10.1080/00207450802330819
DO - 10.1080/00207450802330819
M3 - Article
C2 - 19125375
AN - SCOPUS:58149252099
SN - 0020-7454
VL - 119
SP - 214
EP - 226
JO - International Journal of Neuroscience
JF - International Journal of Neuroscience
IS - 2
ER -