TY - GEN
T1 - Regulation of maternal-fetal heart rates and coupling in mice fetuses
AU - Khandoker, Ahsan H.
AU - Yoshida, Chihiro
AU - Kasahara, Yoshiyuki
AU - Funamoto, Kiyoe
AU - Nakanishi, Kana
AU - Fukase, Miyabi
AU - Kanda, Keiichi
AU - Haroun, Isra
AU - Niizeki, Kyuichi
AU - Kimura, Yoshitaka
N1 - Funding Information:
ACKNOWLEDGMENT This study was partially supported by Khalifa University Internal Research Fund Level 2 awarded to AH Khandoker and Developmental Disorder Data Multi-level Integration Unit of Medical Sciences Innovation Hub Program (MIH), RIKEN. The authors would like to thank Prof K. Niizeki of Yamagata University Japan for the m files of psi and lamda calculation.
Publisher Copyright:
© 2018 IEEE.
PY - 2018/10/26
Y1 - 2018/10/26
N2 - The aim of this preliminary study is to investigate if there is any evidence of maternal-fetal heart rate coupling in mice fetuses and how the coupling patterns are regulated by vagal nervous system on beat by beat. Total of 6 pregnant female mice were divided into two groups [control (N=3) and vagal blockade (N=3)]. On 17.5-day beat-to-beat heart rates of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and atropine (to the vagal blockade group)) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that occasional strong maternal-fetal heart rate coupling (strength was measured by λ appeared and its patterns changed with atropine infusion (no change with saline). Additionally, fisher's exact test shows that changes (increase/decrease from pre to post injection values) in mean, rmssd and power spectral density (PSD) (24 Hz) of MHR, rmssd FHR and PSD (24 Hz) of λ were found to be significantly (p<0.05) associated with treatment types (saline/ atropine). The presented results and protocol allow for the first time in the assessment of autonomic regulation of maternal and fetal heart and their interactions, which will further enhance the value of the mouse as a murine model of heritable human pregnancy and perinatal complications due to maternal conditions.
AB - The aim of this preliminary study is to investigate if there is any evidence of maternal-fetal heart rate coupling in mice fetuses and how the coupling patterns are regulated by vagal nervous system on beat by beat. Total of 6 pregnant female mice were divided into two groups [control (N=3) and vagal blockade (N=3)]. On 17.5-day beat-to-beat heart rates of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and atropine (to the vagal blockade group)) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that occasional strong maternal-fetal heart rate coupling (strength was measured by λ appeared and its patterns changed with atropine infusion (no change with saline). Additionally, fisher's exact test shows that changes (increase/decrease from pre to post injection values) in mean, rmssd and power spectral density (PSD) (24 Hz) of MHR, rmssd FHR and PSD (24 Hz) of λ were found to be significantly (p<0.05) associated with treatment types (saline/ atropine). The presented results and protocol allow for the first time in the assessment of autonomic regulation of maternal and fetal heart and their interactions, which will further enhance the value of the mouse as a murine model of heritable human pregnancy and perinatal complications due to maternal conditions.
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U2 - 10.1109/EMBC.2018.8513463
DO - 10.1109/EMBC.2018.8513463
M3 - Conference contribution
C2 - 30441524
AN - SCOPUS:85056671055
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 5257
EP - 5260
BT - 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
Y2 - 18 July 2018 through 21 July 2018
ER -