Regulation of Output Current of L-Lactate Sensors Based on Alternate Deposition of Avidin and Biotinylated Lactate Oxidase on Electrode Surface Through Avidin/Biotin Complexation

Jun ichi Anzai; Hiroki Takeshita; Tomonori Hoshi; Tetsuo Osa

doi:10.1248/cpb.43.520

Regulation of Output Current of L-Lactate Sensors Based on Alternate Deposition of Avidin and Biotinylated Lactate Oxidase on Electrode Surface Through Avidin/Biotin Complexation

Jun ichi Anzai, Hiroki Takeshita, Tomonori Hoshi, Tetsuo Osa

HOSP - Clinical Research, Innovation and Education Center

Tohoku University

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

An alternate and repeated deposition of avidin and biotinylated lactate oxidase (LOx) gave a thin film on the surface of platinum electrode. Amperometric response of L-lactate sensors thus prepared was enhanced stepwise by increasing the number of LOx layers. The enhanced response contributed to the extension of the lower detection limit of the sensor. The response time of the sensors was satisfactorily fast (ca. 10 s), irrespective of the number of LOx layers.

Original language	English
Pages (from-to)	520-522
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	43
Issue number	3
DOIs	https://doi.org/10.1248/cpb.43.520
Publication status	Published - 1995

Keywords

avidin/biotin system
L-lactate
lactate oxidase
protein multilayer
sensor

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10.1248/cpb.43.520

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abstract = "An alternate and repeated deposition of avidin and biotinylated lactate oxidase (LOx) gave a thin film on the surface of platinum electrode. Amperometric response of L-lactate sensors thus prepared was enhanced stepwise by increasing the number of LOx layers. The enhanced response contributed to the extension of the lower detection limit of the sensor. The response time of the sensors was satisfactorily fast (ca. 10 s), irrespective of the number of LOx layers.",

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Regulation of Output Current of L-Lactate Sensors Based on Alternate Deposition of Avidin and Biotinylated Lactate Oxidase on Electrode Surface Through Avidin/Biotin Complexation. / Anzai, Jun ichi; Takeshita, Hiroki; Hoshi, Tomonori et al.
In: Chemical and Pharmaceutical Bulletin, Vol. 43, No. 3, 1995, p. 520-522.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Regulation of Output Current of L-Lactate Sensors Based on Alternate Deposition of Avidin and Biotinylated Lactate Oxidase on Electrode Surface Through Avidin/Biotin Complexation

AU - Anzai, Jun ichi

AU - Takeshita, Hiroki

AU - Hoshi, Tomonori

AU - Osa, Tetsuo

PY - 1995

Y1 - 1995

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AB - An alternate and repeated deposition of avidin and biotinylated lactate oxidase (LOx) gave a thin film on the surface of platinum electrode. Amperometric response of L-lactate sensors thus prepared was enhanced stepwise by increasing the number of LOx layers. The enhanced response contributed to the extension of the lower detection limit of the sensor. The response time of the sensors was satisfactorily fast (ca. 10 s), irrespective of the number of LOx layers.

KW - avidin/biotin system

KW - L-lactate

KW - lactate oxidase

KW - protein multilayer

KW - sensor

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Regulation of Output Current of L-Lactate Sensors Based on Alternate Deposition of Avidin and Biotinylated Lactate Oxidase on Electrode Surface Through Avidin/Biotin Complexation

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Keywords

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