TY - JOUR
T1 - Reproduction of vesicles coupled with a vesicle surface-confined enzymatic polymerisation
AU - Kurisu, Minoru
AU - Aoki, Harutaka
AU - Jimbo, Takehiro
AU - Sakuma, Yuka
AU - Imai, Masayuki
AU - Serrano-Luginbühl, Sandra
AU - Walde, Peter
N1 - Funding Information:
This work was in part supported by JSPS KAKENHI Grant Numbers JP25247070, JP16H02216, JP17K14368 and JSPS KAKENHI “Fluctuation and Structure” Grant Number JP25103009. We thank Prof. Toshihiro Kawakatsu (Tohoku University) for helpful discussions.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Molecular assembly systems that have autonomous reproduction and Darwinian evolution abilities can be considered as minimal cell-like systems. Here we demonstrate the reproduction of cell-sized vesicles composed of AOT, i.e., sodium bis-(2-ethylhexyl) sulfosuccinate, coupled with an enzymatic polymerisation reaction occurring on the surface of the vesicles. The particular reaction used is the horseradish peroxidase-catalysed polymerisation of aniline with hydrogen peroxide as oxidant, which yields polyaniline in its emeraldine salt form (PANI-ES). If AOT micelles are added during this polymerisation reaction, the AOT - PANI-ES vesicles interact with the AOT molecules in the external solution and selectively incorporate them in their membrane, which leads to a growth of the vesicles. If the AOT vesicles also contain cholesterol, the vesicles not only show growth, but also reproduction. An important characteristic of this reproduction system is that the AOT-based vesicles encourage the synthesis of PANI-ES and PANI-ES promotes the growth of AOT vesicles.
AB - Molecular assembly systems that have autonomous reproduction and Darwinian evolution abilities can be considered as minimal cell-like systems. Here we demonstrate the reproduction of cell-sized vesicles composed of AOT, i.e., sodium bis-(2-ethylhexyl) sulfosuccinate, coupled with an enzymatic polymerisation reaction occurring on the surface of the vesicles. The particular reaction used is the horseradish peroxidase-catalysed polymerisation of aniline with hydrogen peroxide as oxidant, which yields polyaniline in its emeraldine salt form (PANI-ES). If AOT micelles are added during this polymerisation reaction, the AOT - PANI-ES vesicles interact with the AOT molecules in the external solution and selectively incorporate them in their membrane, which leads to a growth of the vesicles. If the AOT vesicles also contain cholesterol, the vesicles not only show growth, but also reproduction. An important characteristic of this reproduction system is that the AOT-based vesicles encourage the synthesis of PANI-ES and PANI-ES promotes the growth of AOT vesicles.
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U2 - 10.1038/s42004-019-0218-0
DO - 10.1038/s42004-019-0218-0
M3 - Article
AN - SCOPUS:85073542368
SN - 2399-3669
VL - 2
JO - Communications Chemistry
JF - Communications Chemistry
IS - 1
M1 - 117
ER -