TY - JOUR
T1 - Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I
AU - Ogawa, Yohsuke
AU - Sugiura, Keita
AU - Kato, Kentaro
AU - Tohya, Yukinobu
AU - Akashi, Hiroomi
PY - 2007/12
Y1 - 2007/12
N2 - Reverse-genetic systems are often used to study different aspects of the viral life cycle. To date, three rescue systems have been developed for the family Bunyaviridae. These systems use T7 RNA polymerase, which is generally used in rescue systems for Mononegavirates. In the present study, we describe a rescue system for Akabane virus (family Bunyaviridae) that uses cDNAs and RNA polymerase I instead of T7 RNA polymerase. The utility of this system was demonstrated by the generation of a mutant with a deletion of the non-structural protein (NSs) on the S RNA segment. These results offer a new option for bunyavirus rescue.
AB - Reverse-genetic systems are often used to study different aspects of the viral life cycle. To date, three rescue systems have been developed for the family Bunyaviridae. These systems use T7 RNA polymerase, which is generally used in rescue systems for Mononegavirates. In the present study, we describe a rescue system for Akabane virus (family Bunyaviridae) that uses cDNAs and RNA polymerase I instead of T7 RNA polymerase. The utility of this system was demonstrated by the generation of a mutant with a deletion of the non-structural protein (NSs) on the S RNA segment. These results offer a new option for bunyavirus rescue.
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U2 - 10.1099/vir.0.83173-0
DO - 10.1099/vir.0.83173-0
M3 - Article
C2 - 18024908
AN - SCOPUS:36749039650
SN - 0022-1317
VL - 88
SP - 3385
EP - 3390
JO - Journal of General Virology
JF - Journal of General Virology
IS - 12
ER -