Response of morphology and microbial community structure of granules to influent COD/SO42 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater

Xueqin Lu; Jialing Ni; Guangyin Zhen; Kengo Kubota; Yu You Li

doi:10.1016/j.biortech.2018.02.055

Response of morphology and microbial community structure of granules to influent COD/SO₄^2 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater

Xueqin Lu, Jialing Ni, Guangyin Zhen, Kengo Kubota, Yu You Li

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

Biochemical properties of granules are of vital importance to UASB performance. This study characterized the granules cultivated at different COD/SO₄²⁻ ratios to elucidate the influence of sulfidogenesis on starch wastewater (1000 mg-COD L⁻¹) biodegradation kinetics and process stability. Suitable sulfate addition enriched granular microecosystems and stimulated the secretion of extracellular substances, facilitating cells cohersion and sludge aggregation. The percentage of granules larger than 2.8 mm increased from <10.0% to 58.8–69.4% with decreasing COD/SO₄²⁻ ratio from 10 to 2. Starch-fed granules tended to grow flagella-like filaments on the surface. The filaments overwhelmed by hydrophilic biopolymers had high affinity for biogas-bubbles and water-molecules aggravating granule floatation and washout. 16 s rRNA gene analysis revealed that decreasing COD/SO₄²⁻ ratio shifted Syntrophobacterales to Desulfovibrio, which co-worked with Methanosaeta while suppressing Methanobacterium thereby altering starch bioconversion routes. Decrease in Syntrophobacterales caused propionate accumulation and slight process upset.

Original language	English
Pages (from-to)	456-465
Number of pages	10
Journal	Bioresource Technology
Volume	256
DOIs	https://doi.org/10.1016/j.biortech.2018.02.055
Publication status	Published - 2018 May

Keywords

Extracellular polymeric substances (EPS)
Granules
Microbial community
Starch wastewater
Sulfate reduction

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2018.02.055

Cite this

@article{7ad28cf00e7b4ab38401867e5798317b,

title = "Response of morphology and microbial community structure of granules to influent COD/SO42 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater",

abstract = "Biochemical properties of granules are of vital importance to UASB performance. This study characterized the granules cultivated at different COD/SO42− ratios to elucidate the influence of sulfidogenesis on starch wastewater (1000 mg-COD L−1) biodegradation kinetics and process stability. Suitable sulfate addition enriched granular microecosystems and stimulated the secretion of extracellular substances, facilitating cells cohersion and sludge aggregation. The percentage of granules larger than 2.8 mm increased from <10.0% to 58.8–69.4% with decreasing COD/SO42− ratio from 10 to 2. Starch-fed granules tended to grow flagella-like filaments on the surface. The filaments overwhelmed by hydrophilic biopolymers had high affinity for biogas-bubbles and water-molecules aggravating granule floatation and washout. 16 s rRNA gene analysis revealed that decreasing COD/SO42− ratio shifted Syntrophobacterales to Desulfovibrio, which co-worked with Methanosaeta while suppressing Methanobacterium thereby altering starch bioconversion routes. Decrease in Syntrophobacterales caused propionate accumulation and slight process upset.",

keywords = "Extracellular polymeric substances (EPS), Granules, Microbial community, Starch wastewater, Sulfate reduction",

author = "Xueqin Lu and Jialing Ni and Guangyin Zhen and Kengo Kubota and Li, {Yu You}",

note = "Funding Information: The first author of this study was supported by the postdoctoral fellowship (ID No. P 16352) of the Japan Society for the Promotion of Science. Guangyin Zhen acknowledges the financial support from the Science & Technology Innovation Action Plan of Shanghai under the Belt and Road Initiative (17230741100), the Distinguished Professor in Universities of Shanghai (Oriental Scholar), the Shanghai Pujiang Program (17PJ1402100), and the Fundamental Research Funds for the Central Universities (40500-20101-222001). This research work was supported by Grant-in-Aid for JSPS Research Fellow (Number 16F16352). Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2018",

month = may,

doi = "10.1016/j.biortech.2018.02.055",

language = "English",

volume = "256",

pages = "456--465",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Response of morphology and microbial community structure of granules to influent COD/SO42 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater

AU - Lu, Xueqin

AU - Ni, Jialing

AU - Zhen, Guangyin

AU - Kubota, Kengo

AU - Li, Yu You

N1 - Funding Information: The first author of this study was supported by the postdoctoral fellowship (ID No. P 16352) of the Japan Society for the Promotion of Science. Guangyin Zhen acknowledges the financial support from the Science & Technology Innovation Action Plan of Shanghai under the Belt and Road Initiative (17230741100), the Distinguished Professor in Universities of Shanghai (Oriental Scholar), the Shanghai Pujiang Program (17PJ1402100), and the Fundamental Research Funds for the Central Universities (40500-20101-222001). This research work was supported by Grant-in-Aid for JSPS Research Fellow (Number 16F16352). Publisher Copyright: © 2018 Elsevier Ltd

PY - 2018/5

Y1 - 2018/5

N2 - Biochemical properties of granules are of vital importance to UASB performance. This study characterized the granules cultivated at different COD/SO42− ratios to elucidate the influence of sulfidogenesis on starch wastewater (1000 mg-COD L−1) biodegradation kinetics and process stability. Suitable sulfate addition enriched granular microecosystems and stimulated the secretion of extracellular substances, facilitating cells cohersion and sludge aggregation. The percentage of granules larger than 2.8 mm increased from <10.0% to 58.8–69.4% with decreasing COD/SO42− ratio from 10 to 2. Starch-fed granules tended to grow flagella-like filaments on the surface. The filaments overwhelmed by hydrophilic biopolymers had high affinity for biogas-bubbles and water-molecules aggravating granule floatation and washout. 16 s rRNA gene analysis revealed that decreasing COD/SO42− ratio shifted Syntrophobacterales to Desulfovibrio, which co-worked with Methanosaeta while suppressing Methanobacterium thereby altering starch bioconversion routes. Decrease in Syntrophobacterales caused propionate accumulation and slight process upset.

AB - Biochemical properties of granules are of vital importance to UASB performance. This study characterized the granules cultivated at different COD/SO42− ratios to elucidate the influence of sulfidogenesis on starch wastewater (1000 mg-COD L−1) biodegradation kinetics and process stability. Suitable sulfate addition enriched granular microecosystems and stimulated the secretion of extracellular substances, facilitating cells cohersion and sludge aggregation. The percentage of granules larger than 2.8 mm increased from <10.0% to 58.8–69.4% with decreasing COD/SO42− ratio from 10 to 2. Starch-fed granules tended to grow flagella-like filaments on the surface. The filaments overwhelmed by hydrophilic biopolymers had high affinity for biogas-bubbles and water-molecules aggravating granule floatation and washout. 16 s rRNA gene analysis revealed that decreasing COD/SO42− ratio shifted Syntrophobacterales to Desulfovibrio, which co-worked with Methanosaeta while suppressing Methanobacterium thereby altering starch bioconversion routes. Decrease in Syntrophobacterales caused propionate accumulation and slight process upset.

KW - Extracellular polymeric substances (EPS)

KW - Granules

KW - Microbial community

KW - Starch wastewater

KW - Sulfate reduction

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DO - 10.1016/j.biortech.2018.02.055

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AN - SCOPUS:85042717382

SN - 0960-8524

VL - 256

SP - 456

EP - 465

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Response of morphology and microbial community structure of granules to influent COD/SO₄^2 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater

Abstract

Keywords

UN SDGs

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Cite this

Response of morphology and microbial community structure of granules to influent COD/SO42 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this

Response of morphology and microbial community structure of granules to influent COD/SO₄^2 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater