TY - JOUR
T1 - Restoration from polyglutamine toxicity after free electron laser irradiation of neuron-like cells
AU - Mohara, Miho
AU - Kawasaki, Takayasu
AU - Owada, Ryuji
AU - Imai, Takayuki
AU - Kanetaka, Hiroyasu
AU - Izumi, Shin ichi
AU - Tsukiyama, Koichi
AU - Nakamura, Kazuhiro
N1 - Funding Information:
This work was supported in part by JSPS KAKENHI Grant Number JP16K15553 (to K. N.) and the Open Advanced Research Facilities Initiative and Photon Beam Platform Project of the Ministry of Education, Culture, Sport, Science and Technology, Japan (to K. T.).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/10/15
Y1 - 2018/10/15
N2 - Proteins containing an expanded polyglutamine tract tend to aggregate, leading to the neuronal damage observed in polyglutamine diseases. We recently reported that free electron laser (FEL) irradiation markedly dissociates naked polyglutamine aggregates as well as the aggregate in the 293 T cells. In the present study, we investigated whether FEL irradiation of neuron-like cells with polyglutamine aggregates would restore the cellular damage and dysfunction. The aggregated polyglutamine peptides induced neurite retraction of differentiated SH-SY5Y cells. Upon FEL irradiation, the polyglutamine aggregates in the SH-SY5Y cells were dissociated, and the shorter length of individual neurite, fewer number of neurites per cell and shorter total length of neurite by polyglutamine were inhibited. Same results were essentially obtained in PC12 cells. Moreover, when FEL irradiation was applied to undifferentiated SH-SY5Y cells, the deficits in neuron-like differentiation seen in expanded polyglutamine peptide-containing cells were also rescued. Thus, FEL irradiation restored both the damage and differentiation caused by polyglutamine in neuron-like cells.
AB - Proteins containing an expanded polyglutamine tract tend to aggregate, leading to the neuronal damage observed in polyglutamine diseases. We recently reported that free electron laser (FEL) irradiation markedly dissociates naked polyglutamine aggregates as well as the aggregate in the 293 T cells. In the present study, we investigated whether FEL irradiation of neuron-like cells with polyglutamine aggregates would restore the cellular damage and dysfunction. The aggregated polyglutamine peptides induced neurite retraction of differentiated SH-SY5Y cells. Upon FEL irradiation, the polyglutamine aggregates in the SH-SY5Y cells were dissociated, and the shorter length of individual neurite, fewer number of neurites per cell and shorter total length of neurite by polyglutamine were inhibited. Same results were essentially obtained in PC12 cells. Moreover, when FEL irradiation was applied to undifferentiated SH-SY5Y cells, the deficits in neuron-like differentiation seen in expanded polyglutamine peptide-containing cells were also rescued. Thus, FEL irradiation restored both the damage and differentiation caused by polyglutamine in neuron-like cells.
KW - Free electron laser
KW - Neurite
KW - Polyglutamine disease
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U2 - 10.1016/j.neulet.2018.07.031
DO - 10.1016/j.neulet.2018.07.031
M3 - Article
C2 - 30044955
AN - SCOPUS:85050643727
SN - 0304-3940
VL - 685
SP - 42
EP - 49
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -