TY - JOUR
T1 - RET finger protein enhances MBD2- and MBD4-dependent transcriptional repression
AU - Fukushige, Shinichi
AU - Kondo, Emiko
AU - Gu, Zhaodi
AU - Suzuki, Hideyuki
AU - Horii, Akira
N1 - Funding Information:
We thank Dr. B.L.S. Pierce (a professor with the University of Maryland University College) for editorial work in the preparation of this manuscript. This work was supported by Grants-in-Aid from the Ministries of Education, Culture, Sports, Science and Technology, and Health, Labour and Welfare of Japan.
PY - 2006/12/8
Y1 - 2006/12/8
N2 - We recently demonstrated that MBD4 possesses the ability to repress transcription through methyl-CpG and is associated with methylated promoters in the CDKN2A and MLH1 genes. In order to further investigate the role of MBD4 in methylation-based transcriptional repression, a yeast two-hybrid screening was performed, and the RET finger protein (RFP) was found to be one of the major proteins that interact with the transcriptional repression domain in MBD4. The effect of the MBD4-mediated transcriptional repression in methylated CDKN2A and MLH1 promoters was extremely enhanced by the overexpression of RFP. Furthermore, RFP forms a protein complex not only with MBD4 but also with MBD2 or MBD3 and was shown to enhance transcriptional repression through MBD2. These results suggest that RFP is a mediator connecting several MBD proteins and allowing the formation of a more potent transcriptional repressor complex. Because RFP has been detected at high levels in a variety of tumor cell lines as well as testis, and embryos, RFP may have an important role in the enhancement of transcriptional repression through MBD proteins in tumorigenesis, spermatogenesis, and embryogenesis.
AB - We recently demonstrated that MBD4 possesses the ability to repress transcription through methyl-CpG and is associated with methylated promoters in the CDKN2A and MLH1 genes. In order to further investigate the role of MBD4 in methylation-based transcriptional repression, a yeast two-hybrid screening was performed, and the RET finger protein (RFP) was found to be one of the major proteins that interact with the transcriptional repression domain in MBD4. The effect of the MBD4-mediated transcriptional repression in methylated CDKN2A and MLH1 promoters was extremely enhanced by the overexpression of RFP. Furthermore, RFP forms a protein complex not only with MBD4 but also with MBD2 or MBD3 and was shown to enhance transcriptional repression through MBD2. These results suggest that RFP is a mediator connecting several MBD proteins and allowing the formation of a more potent transcriptional repressor complex. Because RFP has been detected at high levels in a variety of tumor cell lines as well as testis, and embryos, RFP may have an important role in the enhancement of transcriptional repression through MBD proteins in tumorigenesis, spermatogenesis, and embryogenesis.
KW - MBD2
KW - MBD4
KW - RFP
KW - Transcriptional repression
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U2 - 10.1016/j.bbrc.2006.10.005
DO - 10.1016/j.bbrc.2006.10.005
M3 - Article
C2 - 17049487
AN - SCOPUS:33750345188
SN - 0006-291X
VL - 351
SP - 85
EP - 92
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -