@inbook{8ba0ba67cf3e40bc86621068f69a0bf5,
title = "Reverse Genetic Analysis of Antiviral Resistance Signaling and the Resistance Mechanism in Arabidopsis thaliana",
abstract = "Antiviral RNA silencing and the resistance gene-conferred defense response are major antiviral immune systems in plants. Several of the components involved have been genetically or biochemically identified in Arabidopsis thaliana. One powerful tool to dissect antiviral immune systems involves a reverse genetic approach that analyzes Arabidopsis mutant lines with impaired antiviral defense responses. In particular, to better understand the signaling networks involved in the resistance gene-conferred antiviral response in host plants, establishment of mutant lines carrying the homozygous mutant allele and antiviral resistance gene is required. The information on well-characterized defense-related signaling mutant alleles and the PCR-based genotyping method provided in this chapter allows the efficient selection of Arabidopsis mutant lines that can be used to study antiviral resistance signaling networks and resistance mechanisms.",
keywords = "Arabidopsis thaliana, Cucumber mosaic virus, Nucleotide-binding and leucine-rich repeat (NB-LRR) protein, R gene, Reverse genetics",
author = "Yukiyo Sato and Hideki Takahashi",
note = "Funding Information: This work was supported by grants for JSPS Research Fellows (15J01964), for “Scientific Research on Innovative Areas” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Grant numbers: 16H06429, 16K21723, and 16H06435), and by the Japan Society for the Promotion of Science (JSPS) through the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of International Agricultural Immunology Research-Core for Quantum Improvement in Food Safety.” Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9635-3_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "27--84",
booktitle = "Methods in Molecular Biology",
}